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首页> 外文期刊>Journal of orthopaedic research >Accumulation of LPS by polyethylene particles decreases bone attachment to implants.
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Accumulation of LPS by polyethylene particles decreases bone attachment to implants.

机译：聚乙烯颗粒对LPS的积累会减少骨骼对植入物的附着。

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Molecules absorbed on the surface of particulate wear debris may contribute to inflammatory reactions that lead to aseptic loosening of implants. Lipopolysaccharide (LPS), a bacterial endotoxin, can attach to many biomaterials and stimulate macrophages to secrete osteoclast-activating cytokines. We tested the adsorption of LPS by polyethylene particles in vitro and examined the biological effects of LPS absorption on bone remodeling around implants in vivo. Polyethylene particles were incubated in radiolabeled LPS solutions, and adsorption of LPS by the particles was quantified by radioassay. Because polyethylene particles are hydrophobic and less dense than water, they floated and clumped when incubated in a water solution of LPS, resulting in low adsorption of LPS. However, when particles were incubated in an ethanol solution of LPS, most of the LPS was adsorbed by the particles, and was resistant to washing with water. Triton X-100 (10%), however, effectively washed the LPS off the particles. In a rat model, the presence of polyethylene particles around the implant in the femoral canal decreased bone attachment to the implant at 6 weeks. Incubating the particles with LPS before implantation, or intermittent administration of LPS systemically, further decreased bone-implant attachment to similar extents, but had no effect on the bone density of the control side femurs. Our data indicate that polyethylene particles have high affinity for LPS, depending on many factors, especially the solvents of the LPS. Intermittent systemic administration of LPS affects bone remodeling but only occurs in the area containing polyethylene particles and titanium implants, supporting the hypothesis that the presence of polyethylene particles around implants can result in accumulation of LPS from exogenous sources. This may cause local levels of LPS that are high enough to affect bone remodeling around implants.
机译：吸收在颗粒磨损碎片表面的分子可能会导致炎症反应，从而导致植入物的无菌性松动。脂多糖（LPS）是一种细菌内毒素，可以附着在许多生物材料上，并刺激巨噬细胞分泌破骨细胞活化细胞因子。我们在体外测试了聚乙烯颗粒对LPS的吸附，并研究了LPS吸收对体内植入物周围骨重塑的生物学影响。将聚乙烯颗粒在放射性标记的LPS溶液中孵育，并通过放射分析定量颗粒对LPS的吸附。由于聚乙烯颗粒是疏水性的，并且密度不如水，因此在LPS水溶液中孵育时，它们会漂浮并结块，导致LPS的吸附性较低。然而，当将颗粒在LPS的乙醇溶液中孵育时，大多数LPS被颗粒吸附，并且耐水洗。但是，Triton X-100（10％）可以有效地将LPS从颗粒中冲洗掉。在大鼠模型中，股骨管中植入物周围的聚乙烯颗粒的存在会在6周时减少骨与植入物的附着。在植入前将颗粒与LPS一起孵育，或系统性地间歇性施用LPS，可以进一步减少骨-植入物的附着程度，但程度相似，但对对照侧股骨的骨密度没有影响。我们的数据表明，取决于许多因素，尤其是LPS的溶剂，聚乙烯颗粒对LPS具有高亲和力。 LPS的间歇性全身给药会影响骨骼重塑，但仅发生在含有聚乙烯颗粒和钛植入物的区域，支持假说：植入物周围聚乙烯颗粒的存在会导致外源性LPS的积累。这可能会导致局部LPS水平过高，从而影响植入物周围的骨骼重塑。

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