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首页> 外文期刊>Journal of orthopaedic research >Genipin crosslinking of cartilage enhances resistance to biochemical degradation and mechanical wear
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Genipin crosslinking of cartilage enhances resistance to biochemical degradation and mechanical wear

机译：软骨素的京尼平交联提高了对生化降解和机械磨损的抵抗力

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Collagen crosslinking enhances many beneficial properties of articular cartilage, including resistance to chemical degradation and mechanical wear, but many crosslinking agents are cytotoxic. The purpose of this study was to evaluate the effectiveness of genipin, a crosslinking agent with favorable biocompatibility and cytotoxicity, as a potential treatment to prevent the degradation and wear of articular cartilage. First, the impact of genipin concentration and treatment duration on the viscoelastic properties of bovine articular cartilage was quantified. Next, two short-term (15min) genipin crosslinking treatments were chosen, and the change in collagenase digestion, cartilage wear, and the friction coefficient of the tissue with these treatments was measured. Finally, chondrocyte viability after exposure to these genipin treatments was assessed. Genipin treatment increased the stiffness of healthy, intact cartilage in a dose-dependent manner. The 15-min crosslinking treatments improved cartilage's resistance to both chemical degradation, particularly at the articular surface, and to damage due to mechanical wear. These enhancements were achieved without sacrificing the low coefficient of friction of the tissue and at a genipin dose that preserved chondrocyte viability. The results of this study suggest that collagen crosslinking via genipin may be a promising preventative treatment to slow the degradation of cartilage. (c) 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1571-1579, 2015.
机译：胶原蛋白交联可增强关节软骨的许多有益特性，包括对化学降解和机械磨损的抵抗力，但许多交联剂具有细胞毒性。这项研究的目的是评估一种具有良好生物相容性和细胞毒性的交联剂-Genipin，作为预防关节软骨退化和磨损的潜在疗法的有效性。首先，定量了Genipin浓度和治疗持续时间对牛关节软骨粘弹性的影响。接下来，选择了两种短期（15分钟）京尼平交联处理，并测量了胶原酶消化，软骨磨损和组织在这些处理下的摩擦系数的变化。最后，评估了暴露于这些Genipin治疗后的软骨细胞活力。 Genipin治疗以剂量依赖性方式增加了健康完整软骨的硬度。 15分钟的交联处理提高了软骨抵抗化学降解（特别是在关节表面处）以及抵抗机械磨损造成的损害的能力。在不牺牲组织的低摩擦系数的情况下，并以能维持软骨细胞活力的Genipin剂量实现了这些增强效果。这项研究的结果表明，通过genipin进行胶原蛋白交联可能是减缓软骨降解的有前途的预防方法。 （c）2015骨科研究学会。由Wiley Periodicals，Inc.出版，J Orthop Res 33：1571-1579，2015。

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