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首页> 外文期刊>Journal of orthopaedic research >No effects of in vivo micro-CT radiation on structural parameters and bone marrow cells in proximal tibia of wistar rats detected after eight weekly scans.
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No effects of in vivo micro-CT radiation on structural parameters and bone marrow cells in proximal tibia of wistar rats detected after eight weekly scans.

机译：每周扫描八次后，发现体内微CT辐射对wistar大鼠胫骨近端结构参数和骨髓细胞没有影响。

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摘要

Recently developed in vivo animal high-resolution micro-CT scanners offer the possibility to monitor longitudinal changes in bone microstructure of small rodents, but may impose high radiation doses that could damage bone tissue. The goal of this study was to determine the effects on the bone of 8 weeks of in vivo scanning of the proximal tibia in female Wistar rats. Eight weekly CT scans were made of the right proximal tibia of nine female, 30-week-old, retired-breeder, Wistar rats. Two weeks after the last weekly scan, a final scan was made. The left leg was only scanned during first and final measurements and served as a control. A two-way ANOVA with repeated measures was performed on the first and last measurements of left and right tibiae for six bone structural parameters. Bone marrow cells were flushed out and tested for cell viability. No significant difference was found between left and right for any of the bone structural parameters (p > 0.05). Structure model index and trabecular separation significantly changed as a result of aging, while none of the other parameters did. No significant difference was found between left and right in absolute and percentage number of cell viability. We did not find any indication that the applied scanning regime, in combination with the particular settings used, would affect the results of in vivo bone structural measurements in long-term studies using aged, female Wistar rats. However, careful consideration should be made when determining the number of scans, particularly when a different experimental design is used.
机译：最近开发的体内动物高分辨率微型CT扫描仪提供了监视小型啮齿动物骨骼微观结构纵向变化的可能性，但可能会施加高剂量的辐射，从而可能损坏骨骼组织。这项研究的目的是确定雌性Wistar大鼠在体内扫描胫骨近端8周后对骨骼的影响。每周进行八次CT扫描，检查了9只30周大的雌性，退休种Wistar大鼠的右胫骨。在最后一次每周扫描后两周，进行了最终扫描。仅在第一次和最终测量期间扫描左腿，并作为对照。对六个胫骨结构参数的左胫骨和右胫骨的第一个和最后一个测量，进行重复测量的双向ANOVA。冲洗出骨髓细胞并测试细胞活力。对于任何骨结构参数，左右之间没有发现显着差异（p> 0.05）。结构模型指数和小梁分离由于老化而显着变化，而其他参数则没有。绝对值和细胞存活率百分比之间在左和右之间没有发现显着差异。在使用老年雌性Wistar大鼠进行的长期研究中，我们没有发现任何迹象表明所应用的扫描方案与所使用的特定设置相结合会影响体内骨骼结构测量的结果。但是，在确定扫描次数时应特别考虑，尤其是在使用其他实验设计时。

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