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首页> 外文期刊>Journal of orthopaedic research >Cartilaginous deposits in subchondral bone in regions of exposed bone in osteoarthritis of the human knee: histomorphometric study of PRG4 distribution in osteoarthritic cartilage.
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Cartilaginous deposits in subchondral bone in regions of exposed bone in osteoarthritis of the human knee: histomorphometric study of PRG4 distribution in osteoarthritic cartilage.

机译：人膝骨关节炎的软骨下骨在软骨下骨中的软骨沉积物：PRG4在骨关节炎软骨中分布的组织形态计量学研究。

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The objective of this study was to identify and characterize cartilaginous deposits aggregates in the subchondral bone in areas of the human osteoarthritic knee with exposed bone. A specific aim was to determine the distribution of the joint lubrication molecule, lubricin/superficial zone protein [referred to by its gene, proteoglycan4 (PRG4)], in these cartilaginous deposits and in osteoarthritic cartilage. This work was carried out in the context of assessing the potential contribution of these chondrocyte aggregates for joint resurfacing in certain cartilage repair procedures. The discarded bone cuts of femoral condyles and tibial plateaus were collected from 11 patients with advanced osteoarthritis (OA) of the knee during total knee arthroplasty; 9 women and 2 men with a mean age of 68 years. Sections of paraffin-embedded tissue were stained with Safranin-O, and with antibodies to type II collagen, alpha-smooth muscle actin (SMA), and PRG4. Chondrocyte aggregates were found in the subchondral bone of regions of exposed bone in sections from five individuals. The average diameter of cartilaginous aggregates was 152 microm, and the average depth of the aggregates below the surface was about 475 microm. Most aggregates were fibrocartilaginous and stained positive for type II collagen. Of interest was the finding that the cartilaginous deposits and osteoarthritic cartilage contained PRG4. Only a small percentage of chondrocytes stained positive for SMA. Cartilaginous deposits containing chondrocyte aggregates exist in subchondral bone in regions of exposed bone in some patients with advanced OA of the knee. These cells may be able to contribute to the resurfacing of the joint in certain cartilage repair procedures.
机译：这项研究的目的是鉴定和表征人骨关节炎膝盖裸露区域的软骨下骨中的软骨沉积物聚集体。一个特定的目标是确定关节润滑分子，润滑素/表层蛋白[由其基因，蛋白聚糖4（PRG4）所指]在这些软骨沉积物和骨关节炎软骨中的分布。这项工作是在评估这些软骨细胞聚集体在某些软骨修复手术中联合铺面的潜在作用的背景下进行的。从11例全膝关节置换术中晚期骨关节炎（OA）患者中收集了股骨con和胫骨平台的废弃骨切口; 9名女性和2名男性，平均年龄为68岁。石蜡包埋的组织切片用Safranin-O以及II型胶原蛋白，α平滑肌肌动蛋白（SMA）和PRG4抗体染色。在五个人的切片中，在裸露骨区域的软骨下骨中发现了软骨细胞聚集体。软骨聚集体的平均直径为152微米，并且在表面以下的聚集体的平均深度为约475微米。大多数聚集体是纤维软骨的，并且对II型胶原染色呈阳性。有趣的发现是软骨沉积物和骨关节炎软骨中含有PRG4。仅有少量的软骨细胞对SMA染色呈阳性。在一些患有晚期膝关节骨性关节炎的患者中，软骨下骨中的软骨沉积物存在于软骨下骨的裸露骨区域。这些细胞可能在某些软骨修复程序中有助于关节的表面重塑。

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