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首页> 外文期刊>Journal of orthopaedic research >Cationic contrast agents improve quantification of glycosaminoglycan (GAG) content by contrast enhanced CT imaging of cartilage.
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Cationic contrast agents improve quantification of glycosaminoglycan (GAG) content by contrast enhanced CT imaging of cartilage.

机译：阳离子造影剂通过增强造影剂的软骨CT成像来改善糖胺聚糖（GAG）含量的定量。

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摘要

Minimally invasive and non-destructive methods to quantify glycosaminoglycans (GAGs) in articular cartilage extracellular matrix are of significant interest for the biochemical analysis of cartilage and diagnosis and tracking of osteoarthritis in vivo. Here, we report the use of cationic iodinated contrast agents in comparison to conventional anionic contrast agents for the quantitative monitoring of GAG concentrations with peripheral quantitative computed tomography. Using an ex vivo bovine osteochondral plug model, the cationic contrast agents were evaluated for their ability to distribute into articular cartilage and generate a positive relationship with GAG content. The cationic agents resulted in much higher equilibrium X-ray attenuations in cartilage extracellular matrix (ECM) than anionic agents. Experiments with samples subjected to enzymatic GAG degradation demonstrated that the cationic agents were up to five times more sensitive (p = 0.0001) to changes in GAG content and had a 24% higher correlation (p = 0.002) compared to the anionic agent (R(2) = 0.86, p < 0.0001 compared with R(2) = 0.62, p = 0.004). The natural inhomogeneous distribution of GAGs in the ECM could clearly be identified in undegraded samples.
机译：量化关节软骨细胞外基质中糖胺聚糖（GAGs）的微创和非破坏性方法对于软骨的生化分析以及体内骨关节炎的诊断和追踪具有重大意义。在这里，我们报告了与常规的阴离子造影剂相比，使用碘化碘造影剂与周围的定量计算机断层摄影术定量监测GAG浓度。使用离体牛骨软骨栓塞模型，评估了阳离子造影剂分配到关节软骨中的能力，并与GAG含量产生正相关。阳离子试剂导致软骨细胞外基质（ECM）的平衡X射线衰减比阴离子试剂高得多。用酶GAG降解的样品进行的实验表明，阳离子试剂对GAG含量变化的敏感度提高了五倍（p = 0.0001），并且与阴离子试剂相比，相关性提高了24％（p = 0.002）（R（ 2）= 0.86，p <0.0001，而R（2）= 0.62，p = 0.004）。在未降解的样品中，可以清楚地识别出ECM中GAG的自然不均匀分布。

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