...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of orthopaedic research >Human platelet-rich plasma stimulates migration and chondrogenic differentiation of human subchondral progenitor cells
【24h】

Human platelet-rich plasma stimulates migration and chondrogenic differentiation of human subchondral progenitor cells

机译：富含人血小板的血浆刺激软骨下祖细胞的迁移和软骨分化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In cartilage repair, platelet-rich plasma (PRP) is used in one-step approaches utilizing microfracture and matrix-induced chondrogenesis procedures, bone marrow-derived cell transplantation, or intra-articular injection. The aim of our study was to evaluate the effect of human PRP on the migration and chondrogenic differentiation of human subchondral progenitors. Human progenitors were derived from subchondral cortico-spongious bone (CSP), were analyzed for their migration capacity upon PRP treatment in 96- well chemotaxis assays and cultured in high-density pellet cultures under serum-free conditions in the presence of 5% PRP. Chemotaxis assays showed that 0.1-100% PRP significantly (p < 0.05) stimulate the migration of CSP compared to untreated controls. Histological staining of proteoglycan and immuno-staining of type II collagen indicated that progenitors stimulated with PRP show significantly increased cartilage matrix formation compared to untreated progenitors. Real-time gene expression analysis of typical chondrocyte marker genes as well as osteogenic and adipogenic markers like osteocalcin and fatty acid binding protein showed that PRP induces the chondrogenic differentiation sequence of human progenitors in high-density pellet cultures, while osteogenic or adipogenic differentiation was not evident. These results suggest that human PRP may enhance the migration and stimulate the chondrogenic differentiation of human subchondral progenitor cells known from microfracture.
机译：在软骨修复中，富含血小板的血浆（PRP）用于一步法，该方法利用微骨折和基质诱导的软骨形成程序，骨髓来源的细胞移植或关节内注射。我们研究的目的是评估人类PRP对人类软骨下祖细胞迁移和软骨分化的影响。人类祖细胞来源于软骨下皮层海绵状骨（CSP），在96孔趋化性测定中分析了PRP处理后的迁移能力，并在无血清条件下在5％PRP的条件下在高密度颗粒培养物中进行了培养。化学趋化分析显示，与未处理的对照组相比，0.1-100％的PRP显着（p <0.05）刺激了CSP的迁移。蛋白聚糖的组织学染色和II型胶原蛋白的免疫染色表明，与未处理的祖细胞相比，PRP刺激的祖细胞显示出明显增加的软骨基质形成。对典型软骨细胞标记基因以及成骨和成脂标记（如骨钙素和脂肪酸结合蛋白）的实时基因表达分析表明，PRP诱导高密度颗粒培养中人类祖细胞的成软骨分化序列，而成骨或成脂分化则没有明显。这些结果表明，人PRP可能会增强迁移，并刺激从微骨折中获悉的人软骨下祖细胞的软骨形成分化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号