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首页> 外文期刊>Journal of oral rehabilitation >Inhibition of human pulpal gelatinases (MMP-2 and MMP-9) by zinc oxide cements.
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Inhibition of human pulpal gelatinases (MMP-2 and MMP-9) by zinc oxide cements.

机译：氧化锌水泥对人牙髓明胶酶（MMP-2和MMP-9）的抑制作用。

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The interaction between metal ions and the oral environment is a major subject matter in dental research. Matrix metalloproteinases (MMPs) have been implicated in several pathological and physiological processes such as, periodontal tissue destruction, root caries, dentin calcification and pulpal inflammation. The aim of this work was to test the effect of zinc released from zinc oxide-eugenol (ZOE) cements, on the activity of the major pulpal gelatinolytic MMPs. Pulpal explants were cultured overnight in Dulbecco's Modified Eagle Medium and the activity of secreted enzymes was analysed by gelatin zymography in buffer conditioned with diverse ZOE cements. Phenanthroline, a zinc chelator, was used to revert the inhibition of MMPs caused by zinc. The major gelatinolytic proteinases present in the conditioned media were characterized as MMP-2 and MMP-9 by immunoprecipitation. All ZOE cements inhibited MMPs activity, whereas phenanthroline could partially revert the inhibition caused by plain ZOE and Intermediate Restorative Material (IRM).
机译：金属离子与口腔环境之间的相互作用是牙科研究的主要主题。基质金属蛋白酶（MMPs）已涉及多种病理和生理过程，例如牙周组织破坏，根龋，牙本质钙化和牙髓炎症。这项工作的目的是测试从氧化锌-丁香酚（ZOE）水泥释放的锌对主要牙髓明胶分解MMP活性的影响。在Dulbecco改良的Eagle培养基中培养外植体过夜，并通过明胶酶谱分析在各种ZOE胶浆条件下的缓冲液中分析分泌的酶的活性。菲咯啉是一种锌螯合剂，用于恢复锌引起的MMP抑制作用。通过免疫沉淀将条件培养基中存在的主要明胶分解酶表征为MMP-2和MMP-9。所有ZOE水泥均抑制MMP活性，而菲咯啉可部分恢复纯ZOE和中间修复材料（IRM）引起的抑制作用。

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