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首页> 外文期刊>Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis >HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue: application in pharmacokinetic and biodistribution studies.
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HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue: application in pharmacokinetic and biodistribution studies.

机译：HPLC-FLD方法定量分析纳米颗粒，血浆和组织中的氯铝酞菁：在药代动力学和生物分布研究中的应用。

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摘要

Analytical and bioanalytical methods of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) were developed and validated for the determination of chloroaluminum phthalocyanine in different formulations of polymeric nanocapsules, plasma and livers of mice. Plasma and homogenized liver samples were extracted with ethyl acetate, and zinc phthalocyanine was used as internal standard. The results indicated that the methods were linear and selective for all matrices studied. Analysis of accuracy and precision showed adequate values, with variations lower than 10% in biological samples and lower than 2% in analytical samples. The recoveries were as high as 96% and 99% in the plasma and livers, respectively. The quantification limit of the analytical method was 1.12 ng/ml, and the limits of quantification of the bioanalytical method were 15 ng/ml and 75 ng/g for plasma and liver samples, respectively. The bioanalytical method developed was sensitive in the ranges of 15-100 ng/ml in plasma and 75-500 ng/g in liver samples and was applied to studies of biodistribution and pharmacokinetics of AlClPc.
机译：建立了高效液相色谱-荧光检测法（HPLC-FLD）的分析和生物分析方法，并验证了该方法可用于测定不同聚合物纳米胶囊，血浆和肝脏配方中的氯铝酞菁。用乙酸乙酯提取血浆和均质肝样品，并用酞菁锌作为内标。结果表明，该方法对所研究的所有矩阵均具有线性和选择性。准确性和精密度分析显示了足够的值，生物样品中的变化低于10％，分析样品中的变化低于2％。在血浆和肝脏中的回收率分别高达96％和99％。分析方法的定量限为1.12 ng / ml，生物分析方法的定量限为血浆和肝样品分别为15 ng / ml和75 ng / g。所开发的生物分析方法对血浆中15-100 ng / ml和肝脏样品中75-500 ng / g的灵敏度敏感，可用于研究AlClPc的生物分布和药代动力学。

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