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首页> 外文期刊>Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis >Application of a bridging ELISA for detection of anti-erythropoietin binding antibodies and a cell-based bioassay for neutralizing antibodies in human sera.
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Application of a bridging ELISA for detection of anti-erythropoietin binding antibodies and a cell-based bioassay for neutralizing antibodies in human sera.

机译：桥接ELISA在检测抗促红细胞生成素结合抗体中的应用以及用于中和人血清中抗体的基于细胞的生物测定。

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Although erythropoietin (EPO)-related pure red-cell aplasia (PRCA) is a rare disorder, attention still needs to be paid because underline mechanism of EPO immunogenicity is various and controversial. Among several assay systems for screening of anti-EPO binding antibodies (Abs), we adopted and setup the bridging ELISA using streptavidin-coated plate. To test their neutralizing activities, cell-based neutralizing (NT) bioassay was setup. When we analyzed serum samples by using these two assays, we found two positive results in the two samples. In the sample 1, 411.9 ng/ml of anti-EPO Abs were found and neutralizing activity of 36.2% at 1:5 serum dilution was detected. In the sample 2, 40.5 ng/ml of anti-EPO Abs were found and neutralizing activity of 96.7% was detected. Our results indicate that the higher anti-EPO antibody (Ab) level in a serum does not always lead to the stronger neutralizing activity. This report gives crucial consideration to the needs of establishing clear criteria to link various assay parameters with the onset of PRCA and its progression.
机译：尽管促红细胞生成素（EPO）相关的纯红细胞发育不全（PRCA）是一种罕见的疾病，但由于EPO免疫原性的强调机制多种多样且存在争议，因此仍需引起注意。在用于筛选抗EPO结合抗体（Abs）的几种测定系统中，我们采用链霉亲和素包被的板采用并建立了桥接ELISA。为了测试其中和活性，建立了基于细胞的中和（NT）生物测定法。当我们使用这两种测定法分析血清样品时，在两个样品中发现两个阳性结果。在样品1中，发现了411.9 ng / ml的抗EPO抗体，在1：5的血清稀释度下检测到的中和活性为36.2％。在样品2中，发现40.5 ng / ml的抗EPO抗体，中和活性为96.7％。我们的结果表明，血清中较高的抗EPO抗体（Ab）并不总是导致较强的中和活性。本报告对建立明确标准以将各种测定参数与PRCA的发作及其进展联系起来的需求进行了重要考虑。

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