...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >OXYGEN PENETRATION AND DIFFUSION INTO MYOGLOBIN REVEALED BY QUENCHING OF ZINCPROTOPORPHYRIN IX FLUORESCENCE
【24h】

OXYGEN PENETRATION AND DIFFUSION INTO MYOGLOBIN REVEALED BY QUENCHING OF ZINCPROTOPORPHYRIN IX FLUORESCENCE

机译：锌原卟啉IX荧光猝灭揭示了氧的渗透和扩散成肌红蛋白

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Oxygen quenching experiments were carried out on zincprotoporphyrin IX reconstituted myoglobin (Mb(Fe-->Zn)) at different temperatures and two solvent viscosities. The data were fit to a dynamic model for quenching of fluorophores in protein interiors previously presented (Biophysical J., 45 (1984) 789-794). The parameters associated with the oxygen entry rate (k(+)), exit rate (k(-)), and migration rate( chi) in the protein were obtained at six temperatures and two viscosities (1 and 8 cp), along with the activation enthalpies associated with the above rates(k(+) and k(-)). The partition coefficient (alpha) was calculated at each temperature along with the free energy, Delta G(0), associated with this partition. The rate parameters (k(+), k(-), chi) and the partition coefficient (alpha) have also been determined for the sample in 40% sucrose (8 cp), to evaluate the effect of bulk solvent viscosities on these values. The steady-state Stern-Volmer quenching plot was calculated using the rate parameters obtained from the analysis (of the dynamic model). Comparison of the Stern-Volmer points obtained using the dynamic model and those obtained experimentally showed excellent agreement. [References: 29]
机译：在不同温度和两种溶剂粘度下，对锌原卟啉IX重构的肌红蛋白（Mb（Fe-> Zn））进行了氧淬灭实验。数据适合于先前提出的用于蛋白质内部的荧光团猝灭的动力学模型（Biophysical J.，45（1984）789-794）。在六个温度和两个粘度（1和8 cp）下，获得了与蛋白质中的氧气进入速率（k（+）），出口速率（k（-））和迁移速率（chi）相关的参数，以及与上述速率（k（+）和k（-））相关的激活焓。计算每个温度下的分配系数α以及与该分配相关的自由能Delta G（0）。还已经确定了40％蔗糖（8 cp）中样品的速率参数（k（+），k（-），chi）和分配系数（alpha），以评估本体溶剂粘度对这些值的影响。使用从分析（动态模型）获得的速率参数计算稳态Stern-Volmer淬灭曲线。使用动态模型获得的Stern-Volmer点与通过实验获得的Stern-Volmer点的比较显示出极好的一致性。 [参考：29]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号