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首页> 外文期刊>Journal of periodontal research >Assessment of the regenerative potential of allogeneic periodontal ligament stem cells in a rodent periodontal defect model
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Assessment of the regenerative potential of allogeneic periodontal ligament stem cells in a rodent periodontal defect model

机译：啮齿动物牙周缺损模型中同种异体牙周膜干细胞再生潜力的评估

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Background and Objective: The complex microenvironment of the periodontal wound creates many challenges associated with multitissue regeneration of periodontal lesions. Recent characterization of mesenchymal stem cell-like populations residing in periodontal ligament tissues has shown that these cells exhibit features of postnatal stem cells. Despite these advances, a lack of consistency in design of preclinical studies and a limited study of allogeneic transplantation applications has restricted our understanding of their clinical utility in the treatment of periodontal disease. The aim of this study was to assess the regenerative potential of allogeneic periodontal ligament stem cells (PDLSCs) in a rat periodontal fenestration defect mode and to identify an optimal end time-point suitable for quantitative assessment of tissue regeneration. Material and Methods: Periodontal fenestration defects, created in Sprague Dawley rats, were treated with allogeneic PDLSCs seeded onto Gelfoam? (Absorbable gelatin sponge; Pharmacia Corporation, Kalamazoo, MI, USA) or with Gelfoam? alone, or remained untreated. Experimental rats were killed at 7, 14, 21 or 28 d after surgery and the tissues were processed for immunohistochemical and histomorphometric examination. Results: Defects treated with PDLSCs showed significantly greater percentage bone fill and length of new bone bridge compared with the untreated group or the group treated withGelfoam? alone on days 14 and 21. Similarly, a statistically significant difference was achieved within specimens retrieved on day 21 for analysis of regeneration of cementum/periodontal ligament (PDL)-like structures. Conclusion: The present investigation shows that allogeneic PDLSCs have a marked ability to repair periodontal defects by forming bone, PDL and cementum-like tissue in vivo. The results suggest that treatment periods of 14 and 21 d are optimal end time-points for quantitative assessment of periodontal regeneration within the rodent fenestration-defect model utilized in the present study.
机译：背景与目的：牙周伤口复杂的微环境给牙周病变的多组织再生带来了许多挑战。居住在牙周膜组织中的间充质干细胞样种群的最新特征表明，这些细胞具有出生后干细胞的特征。尽管取得了这些进展，但临床前研究设计的缺乏一致性以及同种异体移植应用的有限研究限制了我们对其在牙周疾病治疗中的临床实用性的理解。这项研究的目的是评估在大鼠牙周开窗缺损模式下异体牙周膜韧带干细胞（PDLSCs）的再生潜力，并确定适合定量评估组织再生的最佳终点。材料和方法：用异体PDLSC植入Gelfoam？上处理Sprague Dawley大鼠中产生的牙周开窗缺陷。 （可吸收的明胶海绵;美国密歇根州卡拉马祖市的Pharmacia Corporation）还是与Gelfoam？还是不予治疗。实验大鼠在手术后第7、14、21或28天被处死，并对其组织进行免疫组织化学和组织形态学检查。结果：与未治疗组或接受Gelfoam？治疗的组相比，用PDLSC治疗的缺损显示出更大的骨填充百分比和新的骨桥长度。仅在第14天和第21天单独进行。相似地，在第21天取回的用于分析牙骨质/牙周膜韧带（PDL）样结构再生的标本中，在统计学上存在显着差异。结论：目前的研究表明，同种异体PDLSC具有通过在体内形成骨，PDL和牙骨质样组织来修复牙周缺损的显着能力。结果表明，在本研究中使用的啮齿动物开窗-缺损模型中，治疗期14和21 d是定量评估牙周再生的最佳终点。

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