...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of periodontal research >Differential expression of periodontal ligament-specific markers and osteogenic differentiation in human papilloma virus 16-immortalized human gingival fibroblasts and periodontal ligament cells.
【24h】

Differential expression of periodontal ligament-specific markers and osteogenic differentiation in human papilloma virus 16-immortalized human gingival fibroblasts and periodontal ligament cells.

机译：人乳头瘤病毒16永生化人牙龈成纤维细胞和牙周膜细胞中牙周膜特异性标志物的差异表达和成骨分化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background and Objective: Periodontal ligament cells and gingival fibroblasts are important in the remodeling of periodontal tissue, but human papilloma virus (HPV)16-immortalized cell lines derived from human periodontal ligament cells and gingival fibroblasts has not been characterized. The purpose of this study was to establish and differentially characterize the immortalized cell lines from gingival fibroblasts and periodontal ligament by HPV16 transfection. Material and Methods: Cell growth, cell cycle analysis, western blot for cell cycle regulatory proteins and osteogenic differentiation markers, and reverse transcription-polymerase chain reaction for periodontal ligament-specific markers were performed. Results: Both immortalized cell lines (immortalized gingival fibroblasts and immortalized periodontal ligament cells) grew faster than primary cultured gingival fibroblasts or periodontal ligament cells. Immortalized gingival fibroblasts and immortalized periodontal ligament cells overexpressed proteins p16 and p21, and exhibited degradation of proteins pRb and p53, which normally cause cell cycle arrest in G(2)/M-phase. Western blotting and reverse transcription-polymerase chain reaction for periodontal ligament-specific and osteogenic differentiation marker studies demonstrated that a cell line, designated IPDL, mimicked periodontal ligament gene expression for alkaline phosphatase, osteonectin, osteopontin, bone sialoprotein, bone morphogenic protein-2, periostin, S-100A4 and PDLs17. Conclusion: These results indicate that IPDL and immortalized gingival fibroblast cell lines consistently retain normal periodontal ligament and gingival fibroblast phenotypes, respectively, and periodontal ligament markers and osteogenic differentiation in IPDL are distinct from immortalized gingival fibroblast cells.
机译：背景与目的：牙周膜细胞和牙龈成纤维细胞在牙周组织的重塑中起着重要的作用，但是人乳头瘤病毒（HPV）16永生化的人牙周膜细胞和牙龈成纤维细胞的细胞系尚未被鉴定。这项研究的目的是通过HPV16转染建立并区分牙龈成纤维细胞和牙周膜永生化细胞系。材料与方法：进行细胞生长，细胞周期分析，细胞周期调节蛋白和成骨分化标记的蛋白质印迹，以及牙周膜特异性标记的逆转录聚合酶链反应。结果：永生化细胞系（永生化牙龈成纤维细胞和永生化牙周膜细胞）的生长均快于原代培养的牙龈成纤维细胞或牙周膜细胞。永生的牙龈成纤维细胞和永生的牙周膜细胞过度表达蛋白质p16和p21，并表现出蛋白质pRb和p53的降解，通常会导致细胞周期停滞在G（2）/ M期。 Western blotting和逆转录聚合酶链反应用于牙周膜特异性和成骨分化标志物的研究表明，命名为IPDL的细胞系模仿了碱性磷酸酶，骨连接蛋白，骨桥蛋白，骨唾液蛋白，骨形态发生蛋白2的牙周膜基因表达，骨膜素，S-100A4和PDLs17。结论：这些结果表明，IPDL和永生化牙龈成纤维细胞系分别分别保持正常的牙周膜和牙龈成纤维细胞表型，并且IPDL中的牙周膜标记和成骨分化与永生化牙龈成纤维细胞不同。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号