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首页> 外文期刊>Journal of periodontal research >Areca nut extracts increased expression of inflammatory cytokines, tumor necrosis factor-alpha, interleukin-1beta, interleukin-6 and interleukin-8, in peripheral blood mononuclear cells.
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Areca nut extracts increased expression of inflammatory cytokines, tumor necrosis factor-alpha, interleukin-1beta, interleukin-6 and interleukin-8, in peripheral blood mononuclear cells.

机译：槟榔提取物在外周血单核细胞中增加了炎性细胞因子，肿瘤坏死因子-α，白介素-1β，白介素-6和白介素8的表达。

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BACKGROUND AND OBJECTIVE: Cytokines represent a central role in inflammatory tissue destruction and regulate the immune responses that may govern the progression of periodontal diseases. This study investigated the effects of areca nut extracts on the expression of inflammatory cytokines, tumor necrosis factor-alpha, interleukin-1beta, interleukin-6 and interleukin-8 in peripheral blood mononuclear cells. The role of oxidative stress of areca nut extracts was also examined using curcumin. MATERIAL AND METHODS: The expression of cytokines in peripheral blood mononuclear cells treated with extracts of ripe areca nut or extracts of tender areca nut was analyzed using enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction. RESULTS: Both extracts of ripe areca nut (< or = 40 microg/mL) and extracts of tender areca nut significantly enhanced the production of tumor necrosis factor-alpha and interleukin-1beta in peripheral blood mononuclear cells in a dose-dependent and time-dependent manner. The kinetics of mRNA expression of both cytokines was also enhanced by areca nut extracts. The stimulatory effects of areca nut extracts on the secretion of tumor necrosis factor-alpha, interleukin-1beta, interleukin-6 and interleukin-8 and on the mRNA expression of tumor necrosis factor-alpha, interleukin-1beta and interleukin-6 at 4 h of incubation were reduced by curcumin (20-50 microm). However, the level of interleukin-8 transcripts was not affected by curcumin. Moreover, interleukin-1beta induction by extracts of tender areca nut, but not by extracts of ripe areca nut, was weakened by 10 microm curcumin. The inhibitory effects of curcumin may vary with different cytokines and with different areca nut extract treatments. CONCLUSION: The complex cytokine profile induced by areca nut extracts-treated peripheral blood mononuclear cells implied the possibility of enhanced local inflammation and altered immune functions by the areca chewing habit. The inhibitory effects of curcumin on cytokine expression suggested that oxidative stress might be involved in areca nut extracts-associated immune alteration.
机译：背景与目的：细胞因子在炎性组织破坏中起着核心作用，并调节可能控制牙周疾病进展的免疫反应。这项研究调查了槟榔提取物对外周血单核细胞中炎性细胞因子，肿瘤坏死因子-α，白介素-1β，白介素-6和白介素8表达的影响。还使用姜黄素检查了槟榔提取物的氧化应激作用。材料与方法：采用酶联免疫吸附法和逆转录-聚合酶链反应分析了成熟槟榔提取物或嫩槟榔提取物处理的外周血单个核细胞中细胞因子的表达。结果：成熟槟榔提取物（≤40μg/ mL）和嫩槟榔提取物均显着增强外周血单个核细胞中肿瘤坏死因子-α和白介素-1β的生成，且呈剂量依赖性和时间依赖性。依赖方式。槟榔提取物还增强了两种细胞因子的mRNA表达动力学。槟榔提取物对肿瘤坏死因子-α，白介素-1β，白介素-6和白细胞介素8的分泌以及对肿瘤坏死因子-α，白介素-1β和白细胞介素6的mRNA表达有刺激作用。姜黄素（20-50微米）可减少孵育次数。但是，白介素8转录物的水平不受姜黄素的影响。此外，用10微米姜黄素可减弱嫩槟榔提取物对白细胞介素1β的诱导作用，而成熟槟榔提取物对白介素1β的诱导作用则减弱。姜黄素的抑制作用可能随不同的细胞因子和不同的槟榔提取物处理而变化。结论：槟榔提取物处理的外周血单个核细胞可诱导复杂的细胞因子，这暗示槟榔咀嚼习惯可能会增强局部炎症并改变免疫功能。姜黄素对细胞因子表达的抑制作用提示氧化应激可能与槟榔提取物相关的免疫改变有关。

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