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首页> 外文期刊>Journal of Photochemistry and Photobiology, B. Biology: Official Journal of the European Society for Photobiology >Reduction in hypericin-induced phototoxicity by Hypericum perforatum extracts and pure compounds
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Reduction in hypericin-induced phototoxicity by Hypericum perforatum extracts and pure compounds

机译：贯叶连翘提取物和纯化合物减少金丝桃素诱导的光毒性

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摘要

Clinical evidence suggests that administration of Hypericum perforatum (Hp) extracts containing the photo-activated hypericin compounds may cause fewer skin photo sensitization reactions than administration of pure hypericin. This study was conducted to determine whether the phototoxicity of hypericin in HaCaT keratinocytes could be attenuated by H. perforatum extracts and constituents. Two extracts, when supplemented with 20 mu M hypericin: (1) an ethanol re-extraction of residue following a chloroform extraction (denoted ethanol(-chloroform)) (3.35 mu M hypericin and 124.0 mu M total flavonoids); and (2) a chloroform extract (hypericin and flavonoids not detected), showed 25% and 50% (p < 0.0001) less phototoxicity than 20 mu M hypericin alone. Two H. perforatum constituents, when supplemented with 20 mu M hypericin: (1) 10 mu M chlorogenic acid; and (2) 0.25 mu M pyropheophorbide, exhibited 24% (P < 0.05) and 40% (p < 0.05) less phototoxicity than 20 mu M hypericin alone. The peroxidation of arachidonic acid was assessed as a measure of oxidative damage by photo-activated hypericin, but this parameter of lipid peroxidation was not influenced by the extracts or constituents. However alpha-tocopherol, a known antioxidant also did not influence the amount of lipid peroxidation induced in this system. These observations indicate that hypericin combined with H. perforatum extracts or constituents may exert less phototoxicity than pure hypericin, but possibly not through a reduction in arachidonic acid peroxidation. (c) 2006 Elsevier B.V. All rights reserved.
机译：临床证据表明，施用含光活化金丝桃素化合物的贯叶金丝桃（Hp）提取物可能比纯金丝桃素的施用引起更少的皮肤光敏反应。进行这项研究来确定贯叶连翘提取物和成分是否能减轻HaCaT角质形成细胞中金丝桃素的光毒性。两种提取物，当补充有20μM金丝桃素时：（1）用氯仿萃取后残留物的乙醇再萃取（表示乙醇（-氯仿））（3.35μM金丝桃素和124.0μM总黄酮）; （2）氯仿提取物（未检出高丝素和类黄酮）显示的光毒性比单独使用20μM金丝桃素低25％和50％（p <0.0001）。当补充20μM金丝桃素时，有两个穿山楂成分：（1）10μM绿原酸; （2）0.25μM焦脱镁叶绿酸比单独使用20μM金丝桃素具有24％（P <0.05）和40％（p <0.05）的光毒性。花生四烯酸的过氧化被评估为光活化金丝桃素对氧化损伤的量度，但是脂质过氧化的该参数不受提取物或成分的影响。但是，α-生育酚（一种已知的抗氧化剂）也不会影响该系统中诱导的脂质过氧化作用的量。这些观察结果表明，金丝桃素与贯叶连翘提取物或成分的组合可能比纯金丝桃素具有较小的光毒性，但可能不是通过降低花生四烯酸过氧化作用来实现的。 （c）2006 Elsevier B.V.保留所有权利。

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