Preparation of core-shell Fe_3O_4/SiO_2 microspheres as adsorbents for purification of DNA

Gai L.; Li Z.; Hou Y.; Jiang H.; Han X.; Ma W.

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Preparation of core-shell Fe_3O_4/SiO_2 microspheres as adsorbents for purification of DNA

机译：核壳Fe_3O_4 / SiO_2微球的制备作为DNA纯化的吸附剂

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Nearly monodisperse core-shell Fe_3O_4/SiO_2 microspheres have been prepared via a glycol reduction method followed by a modified St?ber process. The thickness of the silica shells can be tuned in the range 33-53 nm by varying the amount of tetraethyl silicate (TEOS) during syntheses. The magnetic composite microspheres were characterized with XRD, XPS, FTIR, TEM, ICP-OES and VSM, and further tested as adsorbents for purification of plasmid DNA from Escherichia coli DH5α cells. The magnetic purification of plasmid DNA leads to satisfying integrity, yield and purity in comparison with those isolated by the traditional phenol-chloroform extraction.

机译：通过乙二醇还原法，然后采用改进的Stber方法制备了几乎单分散的核壳型Fe_3O_4 / SiO_2微球。通过在合成过程中改变硅酸四乙酯（TEOS）的量，可以在33-53 nm范围内调整二氧化硅壳的厚度。用XRD，XPS，FTIR，TEM，ICP-OES和VSM对磁性复合微球进行了表征，并进一步测试了其作为吸附剂的能力，可从大肠杆菌DH5α细胞中纯化质粒DNA。与通过传统的苯酚-氯仿提取所分离的DNA相比，对质粒DNA的磁性纯化导致令人满意的完整性，产率和纯度。

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《Journal of Physics, D. Applied Physics: A Europhysics Journal》 |2010年第44期|共8页
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Gai L.; Li Z.; Hou Y.; Jiang H.; Han X.; Ma W.;
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1. Preparation of core-shell Fe_3O_4/SiO_2 microspheres as adsorbents for purification of DNA [J] . Gai L., Li Z., Hou Y., Journal of Physics, D. Applied Physics: A Europhysics Journal . 2010,第44期

机译：核壳Fe_3O_4 / SiO_2微球的制备作为DNA纯化的吸附剂
2. Superparamagnetic core-shell structured microspheres carrying carboxyl groups as adsorbents for purification of genomic DNA [J] . Jiang H., Han X., Li Z., Colloids and Surfaces, A. Physicochemical and Engineering Aspects . 2012,第Null期

机译：带有羧基作为吸附剂的超顺磁性核-壳结构微球，用于纯化基因组DNA
3. Preparation and Characterization of SiO_2/SiO_2 Core-Shell Microspheres as RP-HPLC Stationary Phase [J] . Q. Liu, L.-T. Wang, S.-Q. Dong, Journal of inorganic and organometallic polymers and materials . 2011,第4期

机译：RP-HPLC固定相SiO_2 / SiO_2核壳微球的制备与表征
4. Core-shell Structured Fe_3O_4/PPy Microspheres with High Magnetization for Purification of Plasmid DNA [C] . Haihui Jiang, Yan Zhou, Xiaoyun Han, International Conference on Materials Science Technology . 2013

机译：具有高磁化的核心壳结构Fe_3O_4 / PPY微球，用于纯化质粒DNA
5. Synthesis and Characterization of Carbonized Poly(divinylbenzene) Microspheres for Carbon/Nanodiamond/Polymer-Based Core-Shell Materials and Applications of this Mixed-Mode Phase to High Performance Liquid Chromatography [D] . Hung, Chuan-Hsi 2015

机译：碳/纳米金刚石/聚合物基核壳材料的碳化聚二乙烯基苯微球的合成，表征及该混合模式相在高效液相色谱中的应用
6. Preparation of core–shell structured CaCO3 microspheres as rapid and recyclable adsorbent for anionic dyes [O] . Mengen Zhao, Zhenhua Chen, Xinyan Lv, 2017

机译：制备核壳结构的CaCO3微球作为阴离子染料的快速可回收吸附剂
7. Preparation of Core-Shell SiO_2/Pt Particles via Layer-by-Layer Assembly and Their Electrooxidation and In-situ Electrochemical FTIR Spectra for CO [O] . 李永军, 周宥辰, 孙世刚 2010

机译：层状组装制备核壳SiO_2 / Pt颗粒及其电氧化和CO原位电化学FTIR光谱

Preparation of core-shell Fe_3O_4/SiO_2 microspheres as adsorbents for purification of DNA

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