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首页> 外文期刊>Journal of Physics, D. Applied Physics: A Europhysics Journal >Biomolecular sensing via coupling DNA-based recognition with gold nanoparticles
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Biomolecular sensing via coupling DNA-based recognition with gold nanoparticles

机译:通过基于DNA的识别与金纳米颗粒的耦合进行生物分子传感

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摘要

Gold nanoparticles (AuNPs) have been extensively employed to develop novel nanobiosensorsbased on their unique optical properties. Here we first describe the unique localized surfaceplasmon resonance properties of AuNPs, which can be finely tuned via the aggregation ofAuNPs and disassembly of AuNP aggregates. We then review the applications of bothAuNPs–DNA conjugates and unmodified AuNPs in selective detection of a variety ofmolecular targets, including DNA, proteins, small molecules and ions, by incorporating DNAhybridization or aptamer–target binding. In many situations, target-induced assembly anddisassembly lead to a characteristic red/blue colour change that arises from the shift of surfaceplasmon resonance absorption of AuNPs. The superquenching ability of AuNPs and itsapplication in fluorescent sensors are also discussed.
机译:金纳米粒子(AuNPs)已被广泛用于开发基于其独特的光学特性的新型纳米生物传感器。在这里,我们首先描述AuNPs的独特的局部表面等离子体共振特性,可以通过AuNPs的聚集和AuNP聚集体的分解进行微调。然后,我们通过结合DNA杂交或适体-靶标结合,综述了AuNPs-DNA缀合物和未修饰的AuNPs在选择性检测多种分子靶标(包括DNA,蛋白质,小分子和离子)中的应用。在许多情况下,目标诱导的组装和拆卸会导致特征性的红/蓝颜色变化,这是由于AuNPs的表面等离子体共振吸收的移动引起的。还讨论了AuNPs的超猝灭能力及其在荧光传感器中的应用。

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