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首页> 外文期刊>Journal of Plant Diseases and Protection >Effects of Erysiphe pisi on protein profiles and ribulose-1,5-bisphosphate carboxylase content in resistant and susceptible pea (Pisum sativum x Pisum fulvum) plants.
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Effects of Erysiphe pisi on protein profiles and ribulose-1,5-bisphosphate carboxylase content in resistant and susceptible pea (Pisum sativum x Pisum fulvum) plants.

机译：豌豆对抗性和易感豌豆（Pisum sativum x Pisum fulvum ）蛋白质谱和核糖-1,5-双磷酸羧化酶含量的影响植物。

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摘要

The fate of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was examined in leaves of both resistant and susceptible plants from a Pisum sativum x Pisum fulvum cross after challenge with Erysiphe pisi. High performance liquid chromatography and 1-dimensional gel electrophoresis revealed a 51 kDa protein that was much more abundant in leaves of resistant versus susceptible plants, and which was identified as the Rubisco large chain precursor by mass spectrometry. Greater quantities of Rubisco in resistant tissue suggested that the protein may serve as an important nutrient source for E. pisi in infected susceptible leaves. Liquid chromatography - tandem mass spectrometry revealed three defense-response proteins only in extracts from resistant tissue, while proteins similar to the antimicrobial peptides viscotoxin and phoratoxin from mistletoes, and bubble protein from the yeast Williopsis mrakii, were found only in susceptible tissue. Greater numbers of DNA synthesis and regulation proteins were also identified in the susceptible vs. resistant tissue, probably resulting from changes in host metabolism induced by E. pisi.
机译：攻击后，从 Pisum sativum x Pisum fulvum 杂交的抗性和易感植物的叶片中检查了核糖-1,5-双磷酸羧化酶/加氧酶（Rubisco）的命运和Erysiphe pisi。高效液相色谱法和一维凝胶电泳显示51 kDa蛋白在抗性植物叶片中比易感植物中丰富得多，并且通过质谱鉴定为Rubisco大链前体。抗性组织中大量的Rubisco提示该蛋白质可能是 E的重要营养来源。易感染叶片中的pisi 。液相色谱-串联质谱法仅在抗性组织的提取物中发现了三种防御反应蛋白，而从槲寄生中发现了与抗微生物肽粘菌毒素和phoratoxin类似的蛋白，以及从酵母 Williopsis mrakii 提取的气泡蛋白。仅在易感组织中。在易感性与抗性组织中也鉴定出大量的DNA合成和调控蛋白，这可能是由E诱导的宿主代谢变化所致。 isi 。

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