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首页> 外文期刊>Journal of Plant Physiology >Seed-specific expression of a rice prolamin RP3/GUS chimeric gene in Nicotiana tabacum cv. Wisconsin 38.
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Seed-specific expression of a rice prolamin RP3/GUS chimeric gene in Nicotiana tabacum cv. Wisconsin 38.

机译：水稻醇溶蛋白RP3 / GUS嵌合基因在烟草中的种子特异性表达。威斯康星州38。

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摘要

The promoter region of a rice prolamin RP3 isolated from Oryza sativa cv. Tainung 67 was fused with a bacterial beta-D-glucuronidase (GUS) structural gene and expressed in tobacco cv. Wisconsin 38. A significantly higher GUS activity was found in seeds than in any other tissues of transgenic regenerants. The data suggest a seed-specific expression of the rice RP3 promoter in tobacco. The GUS activity in seeds appeared 6 days after flowering (DAF), and reached a maximum from 9 to 15 DAF. Unlike theendosperm-specific expression of the prolamin promoter/GUS chimaeric gene in transgenic tobacco seeds, RP3 promoter/GUS chimaeric gene was induced in both embryo and endosperm tissues. The GUS activity could also be inherited to T2 seeds of transgenic T1progeny plants. It is concluded that the rice prolamin RP3 promoter can drive seed-specific expression in tobacco.
机译：从稻中分离到的稻谷醇溶蛋白RP3的启动子区。 Tainung 67与细菌β-D-葡萄糖醛酸酶（GUS）结构基因融合，并在烟草简历中表达。威斯康星州38.在种子中发现的GUS活性明显高于转基因再生剂的任何其他组织。数据表明水稻RP3启动子在烟草中的种子特异性表达。种子中的GUS活性在开花后6天（DAF）出现，从9到15 DAF达到最大值。与转基因烟草种子中的醇溶蛋白启动子/ GUS嵌合基因的胚乳特异性表达不同，RP3启动子/ GUS嵌合基因在胚和胚乳组织中均被诱导。 GUS活性也可以遗传给转基因T1后代植物的T2种子。结论是稻谷醇溶蛋白RP3启动子可以驱动烟草中的种子特异性表达。

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