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Tissue-specific expression of AUX1 in maize roots

机译:玉米根中AUX1的组织特异性表达

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摘要

The cDNA of a maize homologue of Arabidopsis AUX1 was isolated. The deduced amino acid sequence of ZmAUX1 showed 73 % identity to the corresponding AtAUX1 and displayed 7 to 10 putative trans-membrane domains. Northern experiments revealed ZmAUX1 expression in the tips of all maize root types, including the primary-, lateral-, lateral seminal- and crown-roots. In situ hybridisation experiments with tissue sections of the primary root showed that ZmAUX1 expression was highly tissue-specific, confined to the endodermal and pericycle cell layers of the primary root along the border of the central cylinder, as well as to the epidermal cell layer. This expression pattern makes ZmAUX1 useful as a new marker for studies of root development in maize.
机译:分离了拟南芥AUX1的玉米同源物的cDNA。推导的ZmAUX1氨基酸序列与相应的AtAUX1具有73%的同一性,并显示7至10个推定的跨膜结构域。北方实验揭示了ZmAUX1在所有玉米根类型的尖端中表达,包括初级,侧根,侧生根和冠根。原发根组织切片的原位杂交实验表明,ZmAUX1表达具有高度组织特异性,仅限于沿着中央圆柱体边界的原发根的内胚层和周生细胞层,以及表皮细胞层。这种表达模式使ZmAUX1可用作研究玉米根系发育的新标记。

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