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首页> 外文期刊>Journal of Plant Physiology >Anthocyanins and glutathione S-transferase activities in response to low temperature and frost hardening in Vaccinium myrtillus (L.)
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Anthocyanins and glutathione S-transferase activities in response to low temperature and frost hardening in Vaccinium myrtillus (L.)

机译:花青素和谷胱甘肽S-转移酶活性响应低温和霜冻变硬越桔(L.)

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摘要

Anthocyanin (Acy) contents and GST activities of bilberry (Voccinium myrtillus L.) were investigated in two experiments conducted in June (Exp. I: active growth) and in August September (Exp. II: beginning of frost hardening) in Northern Finland (65degreesN). Bilberry plants were subjected to +2degreesC and +18degreesC in Exp. I or +5/0degreesC (dayight) and +18/+13degreesC (dayight) in Exp. II. GST activities were assessed using either 1-chloro-2,4-dinitrobenzene (CDNB) or trons-cinnamic acid (tCA) as substrates. We found temperature to have no effect on Acy during either active growth or frost hardening. Acy increased several-fold from active growth to the beginning of frost hardening, but no increment was observed during the development of frost hardening. This suggests a rote of Acy in photoprotection at low temperatures rather than their direct involvement in the development of freezing tolerance. The lack of response of GST activity to frost hardening and to temperature in autumn may indicate an indirect role of GSTs in frost hardening as protective enzymes. GST activity was the same with the two substrates studied (CDNB, tCA), supporting the assumption that GSTs could catalyze reactions with endogenous phenylpropanoids.
机译:在芬兰北部,分别在6月(实验I:活跃生长)和8月9月(实验II:霜冻开始)中进行了两次实验,调查了越桔(Voccinium myrtillus L.)的花色苷(Acy)含量和GST活性。 65度)。越桔植株的Exp温度为+ 2°C和+ 18°C。我或+ 5/0°C(白天/夜晚)和+ 18 / + 13°C(白天/夜晚)二。使用1-氯-2,4-二硝基苯(CDNB)或trons-肉桂酸(tCA)作为底物评估GST活性。我们发现温度在主动生长或霜冻硬化期间都不会影响Acy。从活跃生长到霜冻硬化开始,Acy增加了几倍,但在霜冻硬化的发展过程中未观察到增加。这表明Acy在低温下具有光保护作用,而不是直接参与抗冻性的发展。 GST活性对秋季的霜冻硬化和温度缺乏响应,可能表明GSTs在霜冻硬化中作为保护酶具有间接作用。 GST活性与所研究的两种底物(CDNB,tCA)相同,支持了GST可以催化与内源性苯丙烷反应的假设。

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