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首页> 外文期刊>Journal of Plant Physiology >Wounding, anoxia and cold induce sugarbeet sucrose synthase transcriptional changes that are unrelated to protein expression and activity
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Wounding, anoxia and cold induce sugarbeet sucrose synthase transcriptional changes that are unrelated to protein expression and activity

机译：伤口，缺氧和寒冷诱导甜菜蔗糖合酶的转录变化，与蛋白质表达和活性无关

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摘要

Wounding, anoxia, and cold are often encountered during crop production and postharvest storage of plant products. Although the effect of these stresses on the expression of sucrose synthase, a key enzyme in the carbon metabolism of many storage organs, has been investigated in several starch-accumulating plant organs, little information on their effect on sucrose synthase expression in sucrose-storing organs is available. To determine the effect of wounding, anoxia and cold on a sucrose-storing organ, sugarbeet (Beta vulgaris) roots were wounded, subjected to anoxic conditions, or exposed to cold temperatures, and transcript and protein levels for the organ's two sucrose synthase genes (SBSS1 and SBSS2) and sucrose synthase enzyme activity were determined during 24h and 7d time course experiments. Wounding, anoxia and cold were associated with several-fold changes in sucrose synthase transcript levels. SBSS1 transcript levels were elevated in wounded, anoxic and cold-treated roots; SBSS2 transcript levels were elevated in response to wounding, cold, and short exposures (3-12h) to anoxic conditions and reduced in roots exposed to anoxic conditions for more than 24h. SBSS1 and SBSS2 protein levels, however, exhibited little change in stressed roots, even after 7d. Enzyme activity was also relatively unchanged in stressed roots, except for small activity differences of 1-2d duration that were unrelated to transcriptional changes. The disparity between transcript levels, protein abundance and enzyme activity indicate that SBSS1 and SBSS2 expression in response to wounding, anoxia and cold may be regulated by post-transcriptional mechanisms. The unresponsiveness of sucrose synthase protein levels or enzyme activity to wounding, anoxia and cold questions the importance of this enzyme to stress responses in sugarbeet root.
机译：在农作物生产和植物产品的收获后储存期间，经常会发生伤口，缺氧和寒冷。尽管已经在几个积累淀粉的植物器官中研究了这些胁迫对蔗糖合酶表达的影响，蔗糖合酶是许多贮藏器官碳代谢的关键酶，但有关它们对蔗糖贮藏器官中蔗糖合酶表达影响的信息很少。可用。为了确定伤口，缺氧和寒冷对蔗糖储存器官的影响，将甜菜根（Beta vulgaris）根部受伤，缺氧或暴露于寒冷温度下，并对器官的两个蔗糖合酶基因的转录本和蛋白质水平在24h和7d时间过程实验中确定了SBSS1和SBSS2）以及蔗糖合酶的活性。伤口，缺氧和寒冷与蔗糖合酶转录水平的几倍变化有关。受伤，缺氧和冷处理的根中SBSS1转录水平升高; SBSS2转录物水平因缺氧条件下的伤势，寒冷和短暂暴露（3-12h）而升高，而暴露于缺氧条件下超过24h的根系则降低。然而，即使在7天后，SBSS1和SBSS2蛋白水平的受压根部也几乎没有变化。胁迫根中的酶活性也相对不变，除了1-2d持续时间的小活性差异与转录变化无关。转录水平，蛋白质丰度和酶活性之间的差异表明响应创伤，缺氧和寒冷而引起的SBSS1和SBSS2表达可能受转录后机制的调节。蔗糖合酶蛋白水平或酶活性对伤口，缺氧和寒冷的无反应性质疑该酶对甜菜根胁迫反应的重要性。

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