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首页> 外文期刊>Journal of Plant Research >Cassava root membrane proteome reveals activities during storage root maturation
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Cassava root membrane proteome reveals activities during storage root maturation

机译：木薯根膜蛋白质组揭示了贮藏根成熟过程中的活性

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Cassava (Manihot esculenta Crantz) is one of the most important crops of Thailand. Its storage roots are used as food, feed, starch production, and be the important source for biofuel and biodegradable plastic production. Despite the importance of cassava storage roots, little is known about the mechanisms involved in their formation. This present study has focused on comparison of the expression profiles of cassava root proteome at various developmental stages using two-dimensional gel electrophoresis and LC-MS/MS. Based on an anatomical study using Toluidine Blue, the secondary growth was confirmed to be essential during the development of cassava storage root. To investigate biochemical processes occurring during storage root maturation, soluble and membrane proteins were isolated from storage roots harvested from 3-, 6-, 9-, and 12-month-old cassava plants. The proteins with differential expression pattern were analysed and identified to be associated with 8 functional groups: protein folding and degradation, energy, metabolism, secondary metabolism, stress response, transport facilitation, cytoskeleton, and unclassified function. The expression profiling of membrane proteins revealed the proteins involved in protein folding and degradation, energy, and cell structure were highly expressed during early stages of development. Integration of these data along with the information available in genome and transcriptome databases is critical to expand knowledge obtained solely from the field of proteomics. Possible role of identified proteins were discussed in relation with the activities during storage root maturation in cassava.
机译：木薯（Manihot esculenta Crantz）是泰国最重要的农作物之一。它的贮藏根用作食品，饲料，淀粉的生产，并且是生物燃料和可生物降解塑料生产的重要来源。尽管木薯贮藏根很重要，但对其形成所涉及的机制知之甚少。这项研究集中在使用二维凝胶电泳和LC-MS / MS比较木薯根蛋白质组在不同发育阶段的表达谱。根据使用甲苯胺蓝的解剖学研究，证实在木薯贮藏根的发育过程中，次生生长至关重要。为了研究在贮藏根成熟期间发生的生化过程，从3个月，6个月，9个月和12个月大的木薯植物收获的贮藏根中分离了可溶性蛋白和膜蛋白。分析并鉴定了具有差异表达模式的蛋白质，该蛋白质与8个功能组相关：蛋白质折叠和降解，能量，代谢，次生代谢，应激反应，运输促进，细胞骨架和未分类的功能。膜蛋白的表达谱揭示了参与蛋白折叠和降解，能量和细胞结构的蛋白在发育的早期都高度表达。这些数据以及基因组和转录组数据库中可用信息的整合对于扩展仅从蛋白质组学领域获得的知识至关重要。讨论了与木薯贮藏根成熟过程中的活性有关的鉴定蛋白质的可能作用。

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