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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Interaction of CdSe/ZnS quantum dots with the marine diatom Phaeodactylum tricornutum and the green alga Dunaliella tertiolecta: A biophysical approach
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Interaction of CdSe/ZnS quantum dots with the marine diatom Phaeodactylum tricornutum and the green alga Dunaliella tertiolecta: A biophysical approach

机译：CdSe / ZnS量子点与海洋硅藻Phaeodactylum tricornutum和绿藻杜氏杜氏藻的相互作用：一种生物物理方法

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摘要

In this study, we investigated the interaction of nanoparticles, such as CdSe/ZnS quantum dots (QDs), with the marine diatom Phaeodactylum tricornutum and the green alga Dunaliella tertiolecta, as biological models in the marine environment. Fluorescence kinetics measurements indicated that 30 min after dispersion in seawater QDs lost the 60% of the initial emission intensity, possibly due to the occurrence of aggregation processes. However, the presence of algae seemed to mitigate this effect. By using confocal microscopy, we highlighted the presence of QDs adsorbed on the surface of both algae, but not inside the cells. The toxicity of QDs was evaluated in terms of inhibition of growth rate, oxidative stress, and lipid peroxidation. QDs in the range of 1-2.5 nM gradually inhibited the growth rate of P. tricornutum and increased the oxidative stress, as evinced by the increase in lipid peroxidation, reactive oxygen species (ROS) production and activity of two main antioxidant enzymes (superoxide dismutase and catalase). On the contrary, QDs did not inhibit the growth rate of D. tertiolecta, at most a modest stimulation was observed in the range of 0.5-2 nM, suggesting a hormetic response. No effect in the parameters indicating oxidative stress was observed in the green alga. In conclusion our results showed that the biological effects were species-specific.
机译：在这项研究中，我们研究了纳米粒子（例如CdSe / ZnS量子点（QDs））与海洋硅藻Phaeodactylum tricornutum和绿藻杜氏藻（Dunaliella tertiolecta）的相互作用，作为海洋环境中的生物学模型。荧光动力学测量表明，分散在海水中的量子点30分钟后损失了初始发射强度的60％，这可能是由于聚集过程的发生所致。但是，藻类的存在似乎减轻了这种影响。通过使用共聚焦显微镜，我们突出了两种藻类表面吸附的QD的存在，但细胞内部却没有。通过抑制生长速率，氧化应激和脂质过氧化来评估QD的毒性。 1-2.5 nM范围内的QD逐渐抑制了角果疟原虫的生长速度并增加了氧化应激，这表现为脂质过氧化，活性氧（ROS）产生和两种主要抗氧化酶（超氧化物歧化酶）的增加所证明和过氧化氢酶）。相反，QDs不会抑制D. tertiolecta的生长速度，至多在0.5-2 nM的范围内观察到适度的刺激，表明存在一种仿制反应。在绿藻中未观察到指示氧化应激的参数的影响。总之，我们的结果表明生物学效应是物种特异性的。

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