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首页> 外文期刊>Journal of Pathology: Journal of the Pathological Society of Great Britain and Ireland >Clonality analysis in tumours of women by PCR amplification of X-linked genes.
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Clonality analysis in tumours of women by PCR amplification of X-linked genes.

机译：通过X连锁基因的PCR扩增对女性肿瘤进行克隆性分析。

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摘要

Modifications have been made to two polymerase chain reaction (PCR) methods for clonality analysis based on the inactivation patterns of two highly polymorphic X-linked genes encoding the androgen receptor (AR) and monoamine oxidase A (MAOA). These methods have been used to examine the clonal nature of frozen tissues from 42 tumours and 25 non-tumour controls from female subjects. Unbalanced inactivation patterns of the genes, which indicate monoclonality, were frequently observed in tumours of heterozygous (informative) cases (18/35 = 51.4 per cent for the AR gene, 9/30 = 30 per cent for the MAOA gene, and 21/38 = 55.2 per cent for both). Among 23 informative non-tumour controls, only one (4.3 per cent), a reactive lymph node, showed skewing in the AR gene. Successful detection of monoclonality was found to depend on the proportion of tumour cells in the tissues examined. None of the AR or MAOA informative cases containing less than 50 per cent of tumour cells showed imbalance in inactivation patterns. With more than 50 per cent of tumour cells in the samples, 66.6 per cent (18/27) of AR and 39.1 per cent (9/23) of MAOA informative cases showed allelic imbalance, with a combined frequency of 72.4 per cent (21/29) of both genes. Our results demonstrate that the methods described are useful for clonal analysis of tissue samples from female patients.
机译：基于两种高度多态性X连锁编码雄激素受体（AR）和单胺氧化酶A（MAOA）的X连锁基因的失活模式，对两种聚合酶链式反应（PCR）方法进行了修改，以进行克隆性分析。这些方法已用于检查女性受试者的42个肿瘤和25个非肿瘤对照的冷冻组织的克隆性质。在杂合性（信息性）病例的肿瘤中经常观察到基因失活的失衡模式，表明单克隆性（AR基因为18/35 = 51.4％，MAOA基因为9/30 = 30％，21 / 38 = 55.2％）。在23个信息丰富的非肿瘤对照中，只有一个（4.3％）反应性淋巴结显示出AR基因偏斜。发现单克隆抗体的成功检测取决于所检查组织中肿瘤细胞的比例。包含少于50％肿瘤细胞的AR或MAOA信息丰富的病例均未显示失活模式失衡。样本中有超过50％的肿瘤细胞，有66.6％（18/27）的AR和39.1％（9/23）的MAOA信息性病例显示等位基因失衡，合并频率为72.4％（21） / 29）。我们的结果表明，所述方法可用于对女性患者的组织样本进行克隆分析。

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