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首页> 外文期刊>Journal of Pathology: Journal of the Pathological Society of Great Britain and Ireland >Myofibroblasts of palmar fibromatosis co-express transforming growth factor-alpha and epidermal growth factor receptor.
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Myofibroblasts of palmar fibromatosis co-express transforming growth factor-alpha and epidermal growth factor receptor.

机译：手掌纤维瘤病的成肌纤维细胞共表达转化生长因子-α和表皮生长因子受体。

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摘要

Several studies have shown that different growth factors are involved in the pathogenesis of palmar fibromatosis. The aim of the present study was to investigate whether transforming growth factor alpha (TGF-alpha) and its cellular receptor, epidermal growth factor receptor (EGF-R), are expressed in palmar fibromatosis. Nodules from 20 patients with palmar fibromatosis and control normal palmar fascias were studied by the reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. RT-PCR followed by Southern blotting demonstrated that palmar fibromatosis nodules contained high levels of TGF-alpha and EGF-R messenger RNA (mRNA) transcripts, while normal fascias showed only low levels. Depending on the degree of cellularity and fibrosis, the three following histological phases were recognized in palmar fibromatosis nodules: proliferative, involutional, and residual. Immunohistochemistry, using alpha-smooth muscle actin as a cellular marker for myofibroblasts, revealed that TGF-alpha and EGF-R are co-expressed by myofibroblasts in the highly cellular areas of both proliferative and involutional phases, while they are absent or only focally detectable in the fibroblasts of normal fascia and in hypocellular and fibrotic areas of both involutional and residual phases. The restricted co-expression of TGF-alpha and EGF-R to myofibroblasts, the proliferating cellular component of nodules, suggests that an autocrine and/or juxtacrine growth stimulation by TGF-alpha via the EGF-R may be involved in the pathogenesis of palmar fibromatosis.
机译：几项研究表明，不同的生长因子参与了手掌纤维瘤病的发病机理。本研究的目的是调查手掌纤维瘤病中是否表达转化生长因子α（TGF-α）及其细胞受体表皮生长因子受体（EGF-R）。通过逆转录聚合酶链反应（RT-PCR）和免疫组化研究了20例手掌纤维瘤病和正常掌膜筋膜患者的结节。 RT-PCR和Southern印迹显示，手掌纤维瘤病结节含有高水平的TGF-α和EGF-R信使RNA（mRNA）转录本，而正常筋膜则仅低水平。根据细胞性和纤维化的程度，在手掌纤维瘤病结节中可以识别出以下三个组织学阶段：增生性，渐进性和残余性。免疫组织化学使用α-平滑肌肌动蛋白作为成肌纤维细胞的细胞标记，发现TGF-α和EGF-R在增生期和复性期的高度细胞区域中都由成肌纤维细胞共表达，而缺失或仅可局部检测在正常筋膜的成纤维细胞中以及在融合期和残留期的细胞和纤维化区域中都存在。 TGF-α和EGF-R对成肌纤维细胞（结节中增殖的细胞成分）的共表达受限制，这表明TGF-α通过EGF-R刺激自分泌和/或邻分泌的生长可能与手掌的发病有关纤维瘤病。

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