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首页> 外文期刊>Journal of Pathology: Journal of the Pathological Society of Great Britain and Ireland >Role of calcium chelation in high-temperature antigen retrieval at different pH values.
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Role of calcium chelation in high-temperature antigen retrieval at different pH values.

机译：钙螯合在不同pH值下在高温抗原回收中的作用。

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摘要

Recent work by Shi et al. on the mechanism of high-temperature antigen retrieval has claimed that the antigen retrieval process is pH-dependent, with different antigens benefitting at high or low pH values of antigen retrieval solutions. It has previously been claimed that chelation of Ca2+ at high temperature is an essential feature of the antigen retrieval process. In order to resolve this apparent dichotomy, the relative antigen retrieval effects were analysed using the buffers employed by Shi et al. in both a facilitating and an inhibitory mode. The results show that calcium-related effects are optimal at high pH values and do not operate at very low pH. The relative antigen retrieval effectiveness of hydrochloric acid and its metal halide solutions were also investigated in relation to pH. The results of these experiments showed that whilst HCl alone produced antigen retrieval (AR), it also produced severe tissue damage, which was reduced by the inclusion of inorganic salts. These results suggest that antigen retrieval at low pH may be achieved through the dissociation of Ca2+ complexes by high concentrations of H+ ions and/or the breaking up of cross-links from formalin fixation. Results are also presented to show that chaotropic denaturants such as urea and guanidine hydrochloride also function principally through calcium chelation, whilst detergents have no role to play in high-temperature retrieval.
机译：Shi等人的最新工作。关于高温抗原回收的机理，有人声称抗原回收过程是pH依赖性的，不同的抗原在抗原回收溶液的高或低pH值时都会受益。先前已经声称，在高温下螯合Ca2 +是抗原修复过程的基本特征。为了解决这种明显的二分法，使用Shi等人使用的缓冲液分析了相对抗原回收作用。以促进和抑制方式。结果表明，钙相关的作用在高pH值时最佳，而在低pH值下不会起作用。还研究了盐酸及其金属卤化物溶液的相对抗原回收效率与pH的关系。这些实验的结果表明，尽管单独使用HCl会产生抗原修复（AR），但也会产生严重的组织损伤，这种损伤可通过加入无机盐来减少。这些结果表明，通过高浓度的H +离子使Ca2 +复合物解离和/或通过福尔马林固定破坏交联，可以在低pH值下进行抗原修复。结果还表明，离液型变性剂（例如尿素和盐酸胍）也主要通过钙螯合起作用，而去污剂在高温回收中不起作用。

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