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首页> 外文期刊>Journal of Pure & Applied Microbiology >Laccase: Production and Purification by Pseudomonas aeruginosa
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Laccase: Production and Purification by Pseudomonas aeruginosa

机译：漆酶：铜绿假单胞菌的生产和纯化

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摘要

Laccase is belonging to the oxidases of blue multi-copper and will be participating in polymer degradation, monomer cross-linking and aromatic compounds' ring cleavage. This is distributed widely in fungi and higher plants. This is also present in Deuteromycetes, Basidiomycetes and Ascomycetes and also much abundant in white-rot fungi which are lignin-degrading. Also it is used in synthesizing organic substances, having phenols and amines as typical substrates with reaction products as oligomers anddimers which are derived out of coupling from radical reactive intermediates. In recent time, these enzymes are gaining application in fields like paper and pulp, food and textile industry. Also of late it is used in biofuel cells, biosensors design, andalso a tool for medical diagnostics and an agent for bioremediation in cleaning up certain explosives and pesticides in soil as well as herbicides. This present work is focusing on screening, the process optimization in achieving maximum production withpartial purification in extracellular laccases by Pseudomonas aeruginosa. Purification of laccase through filtrate of bacterium culture is achieved as a greatest action after the utilization of DEAE-Sephadex Affinity chromatography, chromatography (Sephadex) of Gel filtration and precipitation of ammonium sulphate. The experimental results are showing better performance in purification and production of laccase through Pseudomonas Aeruginosa.
机译：漆酶属于蓝色多铜的氧化酶，将参与聚合物降解，单体交联和芳族化合物的环裂解。它广泛分布在真菌和高等植物中。这也存在于氘菌，担子菌和子囊菌中，并且在降解木质素的白腐真菌中也非常丰富。它也用于合成有机物，以苯酚和胺为典型底物，反应产物为低聚物和二聚物，它们是从自由基反应性中间体的偶联中衍生而来的。近年来，这些酶正在造纸和纸浆，食品和纺织工业等领域得到应用。最近，它还用于生物燃料电池，生物传感器设计中，并且还用于医学诊断的工具和用于清除土壤中某些炸药和农药以及除草剂的生物修复剂。这项工作的重点是筛选，通过铜绿假单胞菌部分纯化细胞外漆酶以实现最大产量的工艺优化。通过使用DEAE-Sephadex亲和色谱，凝胶过滤色谱（Sephadex）和硫酸铵沉淀，通过细菌培养滤液纯化漆酶是最大的作用。实验结果表明，通过铜绿假单胞菌纯化和生产漆酶具有更好的性能。

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