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Effects of Culture Conditions on PHB Synthesis by Bacillus subtilis

机译:培养条件对枯草芽孢杆菌合成PHB的影响

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摘要

Poly-beta-hydroxyl butyrate (PHB) is a biodegradable thermoplastic which can be extracted from a wide range of bacteria as intracellular granules. They are used in packaging, medicine and agriculture for a wide range of applications. The aim of the present work was selection of Bacillus subtilis PHB producers. In this study, the Bacillus subtilis from soil were isolated and identified, and their PHB production was determined under different conditions such as incubation time, carbon source and nitrogen source. The results showed that, starch was the best carbon sources with significant effect and was better in PHB production and known as low cost substrates to compare with the other sources. Amongest variety of nitrogen sources, glycine was the most suitable. At last, the best optimum incubation period and starch concentration to PHB production were 48 hours and 5% (v/v) and for glycine were 48 hours and 3% (v/v) respectively. The results showed that the best percent of carbon source in PHB production, was not the highest amount, but the best concentration was that do not lead to limitation of bacteria growth.
机译:聚β-羟基丁酸酯(PHB)是一种可生物降解的热塑性塑料,可以从多种细菌中提取为细胞内颗粒。它们广泛用于包装,医药和农业。本工作的目的是选择枯草芽孢杆菌PHB生产者。在本研究中,从土壤中分离并鉴定了枯草芽孢杆菌,并在不同的条件下(如孵育时间,碳源和氮源)确定了它们的PHB产量。结果表明,淀粉是效果最好的最佳碳源,并且在PHB生产中更好,并且与其他来源相比被称为低成本底物。在各种氮源中,最适合使用甘氨酸。最后,对PHB产生的最佳最佳潜伏期和淀粉浓度分别为48小时和5%(v / v),对于甘氨酸而言分别为48小时和3%(v / v)。结果表明,PHB生产中碳源的最佳百分率不是最高,但最佳浓度是不导致细菌生长受限。

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