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首页> 外文期刊>Journal of Pure & Applied Microbiology >Genetic Diversity of Aspergillus flavus Contamination ofSorghum GrainsAbeer R. M. Abd El- Aziz1,
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Genetic Diversity of Aspergillus flavus Contamination ofSorghum GrainsAbeer R. M. Abd El- Aziz1,

机译：高粱谷物黄曲霉污染的遗传多样性Abeer R. M. Abd El-Aziz1，

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Seventeen Aspergillus flavus isolates were isolated from 48 sorghum grain samples from different localities of Riyadh, in the Saudi Arabia. Potential ability to produce aflatoxins (AFs) Bl, B2, Gl and G2 was studied by HPLC analysis of these AFs in the culture extracts. Ten (59%) A. flavus isolates produced detectable levels of AFs at concentrations ranging from 0.4 to 1.6 /ig/kg. The isolates were classified into four chemotypes based on the ability to produce AFs and sclerotia. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers were used, with the aim of study genetic diversity and discriminate aflatoxigenic from non-aflatoxigenic isolates. RAPD and ISSR analysis revealed a high level of genetic diversity in the A. flavus population and useful for genetic characterization. RAPD and ISSR markers were not suitable to discriminate aflatoxigenic and non-aflatoxigenic isolates, but ISSR primers were better compared to RAPD.
机译：从沙特阿拉伯利雅得不同地区的48个高粱谷物样品中分离出17种黄曲霉分离株。通过HPLC分析培养物提取物中的这些AF，研究了产生黄曲霉毒素（AF）B1，B2，G1和G2的潜在能力。十个（59％）黄曲霉分离株在0.4至1.6 / ig / kg的浓度范围内产生可检测水平的AF。根据产生AF和菌核的能力，将分离物分为四种化学型。使用随机扩增的多态性DNA（RAPD）和简单序列间重复（ISSR）标记，目的是研究遗传多样性并区分黄曲霉毒素和非黄曲霉毒素。 RAPD和ISSR分析显示，黄曲霉种群具有较高的遗传多样性，可用于遗传表征。 RAPD和ISSR标记不适合区分黄曲霉毒素和非黄曲霉毒素，但ISSR引物比RAPD更好。

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