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首页> 外文期刊>Journal of Liquid Chromatography >HPLC DETERMINATION OF A METOCLOPRAMIDE AND ONDANSETRON MIXTURE IN 0.9-PERCENT SODIUM CHLORIDE INJECTION
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HPLC DETERMINATION OF A METOCLOPRAMIDE AND ONDANSETRON MIXTURE IN 0.9-PERCENT SODIUM CHLORIDE INJECTION

机译：高效液相色谱法测定0.9％氯化钠注射液中的甲基溴化物和恩丹西酮混合物

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摘要

A high performance liquid chromatography procedure has been developed for the assay of a metoclopramide and ondansetron mixture in 0.9% sodium chloride injection. The separation and quantitation are achieved on a base deactivated octylsilane column at ambient temperature using a mobile phase of 77:23 v/v 0.01 M phosphate buffer, pH 4 - acetonitrile at a flow rate of 1.0 mL/min with detection of analytes at 273 nm. The separation is achieved within 15-20 min with sensitivity in the ng/mL range for each analyte. The method showed linearity for metoclopramide and ondansetron in the 12.5 - 50 and 5-20 mu g/mL ranges, respectively. Accuracy and precision were in the 1 - 2% and 0.3 - 1.3% ranges, respectively, for both drugs. The limits of detection for metoclopramide and ondansetron were 49 and 20 ng/mL, respectively, based on a signal to noise ratio of 3 and a 20 mu L injection.
机译：已经开发了一种高效液相色谱方法，用于测定0.9％氯化钠注射液中的甲氧氯普胺和恩丹西酮的混合物。分离和定量在室温下在碱灭活的辛基硅烷色谱柱上进行，使用流动相77:23 v / v 0.01 M磷酸盐缓冲液，pH 4-乙腈，流速为1.0 mL / min，并在273处检测到分析物纳米每种分析物的分离在15-20分钟内完成，灵敏度在ng / mL范围内。该方法显示了甲氧氯普胺和恩丹西酮的线性分别在12.5-50和5-20μg / mL范围内。两种药物的准确度和精密度分别在1-2％和0.3-1.3％之间。基于3的信噪比和20μL的进样量，甲氧氯普胺和恩丹西酮的检出限分别为49和20 ng / mL。

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