Application of Slicing of One-Dimensional Gels with Subsequent Slice-by-Slice Mass Spectrometry for the Proteomic Profiling of Human Liver Cytochromes P450

Lisitsa AV; Petushkova NA; Thiele H; Moshkovskii SA; Zgoda VG; Karuzina II; Chernobrovkin AL; Skipenko OG; Archakov AI

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Application of Slicing of One-Dimensional Gels with Subsequent Slice-by-Slice Mass Spectrometry for the Proteomic Profiling of Human Liver Cytochromes P450

机译：一维凝胶切片技术及其随后的逐层质谱分析技术在人肝细胞色素P450蛋白质组学分析中的应用

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Sequential thin slicing of one-dimensional electrophoresis gels followed by slice-by-slice mass spectrometry to allow protein identification was used to produce a proteomic map for cytochromes P450. Parallel MALDI-TOF-MS and LC-MS/MS analyses were performed. Combination of the two MS methods increased the quality of protein identification. We have proposed an efficient approach to obtain a comprehensive profile of drug-metabolizing enzymes in the liver that can be used to differentiate between polymorphic variants of cytochromes P450.

机译：一维电泳凝胶的顺序薄切片，然后进行逐层质谱分析以进行蛋白质鉴定，可用于产生细胞色素P450的蛋白质组图。进行了并行MALDI-TOF-MS和LC-MS / MS分析。两种质谱方法的结合提高了蛋白质鉴定的质量。我们提出了一种有效的方法来获得肝脏中药物代谢酶的全面概况，可用于区分细胞色素P450的多态性变体。

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《Journal of proteome research》 |2010年第1期|共9页
作者
Lisitsa AV; Petushkova NA; Thiele H; Moshkovskii SA; Zgoda VG; Karuzina II; Chernobrovkin AL; Skipenko OG; Archakov AI;
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正文语种 eng
中图分类分子生物学;
关键词
human liver microsomes; cytochrome P450; 1DE/MS; 1DE-proteomic map; slice-by-slice mass spectrometry;

机译：人肝微粒体;细胞色素P450;1DE / MS;1DE蛋白质组图谱;逐层质谱;

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1. Application of Slicing of One-Dimensional Gels with Subsequent Slice-by-Slice Mass Spectrometry for the Proteomic Profiling of Human Liver Cytochromes P450 [J] . Lisitsa AV, Petushkova NA, Thiele H, Journal of proteome research . 2010,第1期

机译：一维凝胶切片技术及其随后的逐层质谱分析技术在人肝细胞色素P450蛋白质组学分析中的应用
2. Mass spectrometry-based proteomic analysis of human liver cytochrome(s) P450 [J] . ShrivasK., MindayeS.T., Getie-KebtieM., Toxicology and Applied Pharmacology . 2013,第1期

机译：基于质谱的人肝细胞色素蛋白质组学分析P450
3. Rapid determination of six metabolites from multiple cytochrome P450 probe substrates in human liver microsome by liquid chromatography/mass spectrometry: application to high-throughput inhibition screening of terpenoids [J] . He F, Bi HC, Xie ZY, Rapid Communications in Mass Spectrometry: RCM . 2007,第5期

机译：液相色谱/质谱法快速测定人肝微粒体中多种细胞色素P450探针底物中的六种代谢物：在高通量抑制萜类化合物中的应用
4. High Throughput Laser Diode Thermal Desorption Mass Spectrometry for the Determination of Time-Dependent Cytochrome P450 Inhibition in Human Liver Microsomes [C] . Caroline Gauvin, Renu S. Dhanikula, Huy Khang Vu, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：高通量激光二极管热解吸质谱法测定人肝微粒体中依赖于时间依赖性细胞色素P450的抑制作用
5. Metabolite Profiling of Synthetic Cannabinoids and Identification in Human Blood Via Human Liver Microsome Incubation and High Resolution Tandem Mass Spectrometry [D] . ?Presley, Brandon C. 2020

机译：合成大麻素和鉴定的代谢谱人体血液中通过人肝脏微粒体孵化和高分辨串联质谱法
6. Proteomic profiles of unilateral cryptorchidism in pigs at different ages using MALDI-TOF mass spectrometry and in-gel digestion coupled with mass spectrometry (GeLC-MS/MS) approaches [O] . Nathamon Yimpring, Sittiruk Roytrakul, Janthima Jaresitthikunchai, 2020

机译：使用MALDI-TOF质谱法和凝胶消化与质谱（GELC-MS / MS）接近不同年龄的猪的蛋白质组学谱
7. Detection and enrichment of cytochrome P450s using bespoke affinity chromatography and proteomic techniques. Development of chemical immobilisation and novel affinity chromatography methods, with subsequent proteomic analysis, for the characterisation of cytochrome P450s important in cancer research. [O] . Bateson Hannah 2012

机译：使用定制亲和色谱和蛋白质组学技术检测和富集细胞色素P450。化学固定化和新型亲和色谱方法的发展，以及随后的蛋白质组学分析，用于表征在癌症研究中很重要的细胞色素P450。

Application of Slicing of One-Dimensional Gels with Subsequent Slice-by-Slice Mass Spectrometry for the Proteomic Profiling of Human Liver Cytochromes P450

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