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首页> 外文期刊>Journal of proteomics >A comparative study of venomics of Naja naja from India and Sri Lanka, clinical manifestations and antivenomics of an Indian polyspecific antivenom
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A comparative study of venomics of Naja naja from India and Sri Lanka, clinical manifestations and antivenomics of an Indian polyspecific antivenom

机译：印度和斯里兰卡的眼镜蛇毒液学的比较研究，印度多特异性抗蛇毒血清的临床表现和抗肿瘤作用

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Naja naja (Indian cobra) from Sri Lanka and India is the WHO Category 1 medically important snakes in both countries. Some antivenom produced against Indian N. naja (NNi) were less effective against Sri Lankan N. naja (NNsl). Proteomes of NNi and NNsl venoms were studied by RP-HPLC, SDS-PAGE and LC/MS/MS. Six protein families were identified in both venoms with the most abundant were the 3 finger toxins (3FTs) where cytotoxins (CTX) subtype predominated, followed by phospholipase A(2), cysteine-rich venom protein, snake venom metalloproteases, venom growth factors, and protease inhibitors. Qualitative and quantitative differences in the venomics profiles were observed. Some proteins were isolated from either NNi or NNsl venom. Postsynaptic neurotoxins (NTX) were identified for the first time in NNsl venom. Thus, there are geographic intra-specific variations of venom composition of the two N. naja. The relative abundance of CTX and NTX explained well the clinical manifestations of these venoms. Antivenomics study of an Indian antivenom (Vins) showed the antibodies effectively bound all venom toxins from both snakes but more avidly to the Indian venom proteins. The lower antibody affinity towards the 'heterologous' venom was the likely cause of poor efficacy of the Indian antivenom used to treat NNsl envenoming. (C) 2015 Elsevier B.V. All rights reserved.
机译：来自斯里兰卡和印度的眼镜蛇（印度眼镜蛇）是这两个国家的世界卫生组织1类重要医学蛇。一些针对印度N. naja（NNi）的抗蛇毒药对斯里兰卡N. naja（NNsl）的抗药性较低。通过RP-HPLC，SDS-PAGE和LC / MS / MS研究了NNi和NNsl毒液的蛋白质组。在这两种毒液中鉴定出六个蛋白家族，其中最丰富的是三指毒素（3FTs），其中以细胞毒素（CTX）亚型为主，其次是磷脂酶A（2），富含半胱氨酸的毒蛋白，蛇毒金属蛋白酶，毒液生长因子，和蛋白酶抑制剂。观察到了病毒学特征的定性和定量差异。从NNi或NNsl毒液中分离出一些蛋白质。首次在NNsl毒液中鉴定出突触后神经毒素（NTX）。因此，两个眼镜蛇的毒液成分存在地理上的种内变异。 CTX和NTX的相对丰度很好地解释了这些毒液的临床表现。对印度抗蛇毒素（Vins）的抗病毒学研究表明，该抗体有效结合了两条蛇的所有蛇毒毒素，但更能与印度蛇毒蛋白结合。对“异源”毒液的较低抗体亲和力可能是用于治疗NNsl毒液的印度抗蛇毒药功效低下的原因。 （C）2015 Elsevier B.V.保留所有权利。

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