...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of liquid chromatography and related technologies >Advantages and limitations of derivatization of peptides for improved performance and detectability in capillary isoelectric forcusing (CIEF)
【24h】

Advantages and limitations of derivatization of peptides for improved performance and detectability in capillary isoelectric forcusing (CIEF)

机译：肽衍生化的优点和局限性，以改善毛细管等电forcusing（CIEF）的性能和可检测性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Several proteins of varying molecular weights (Mr) were shown to produce a single species, or multiple species which behaved as a single species, upon analysis with capillary isoelec-tric focusing (cLEF) after derivatization with a large molar excess of the derivatization reagent, 6-aminoquinolyl-N-hydroxysuccin-imidyl carbaniate (AQC). Increased molar excesses of reagent were required as th& molecular weight (Mr) of the sample in-creased. Tbe derivative products exhibited acidic p1 shifts, un-proved peak efficiencies, and lowered (improved) detection limitswhen compared to the native species. In at least one- case, a deriv-ative product (not fully tagged) was shown to exhibit antibody (Ab) recognition when challenged with an Ab, raising the possi-bility of using these derivatives in affinity recognition studies- (e.g., affinity CE, immuno-CE, and. so forth). Problems were encountered with precipitation during derivatization and focus-ing. This problem was more pronounced with the more basic pro-teins. This would appear to limit the’ applicability of this reagent as a universal derivatization reagent for use with clEF studies. The results presented herein represent a promising technique, and they offer advantages as well- as certain’limitations. Though not yet a perfect approach towards improved analysis and identifica-tion of peptides in clEF, these results indicate tangible opportuni-ties for further optimization.
机译：在使用大量摩尔过量的衍生试剂衍生化后，通过毛细管等电聚焦（cLEF）分析后，几种分子量不同（Mr）的蛋白质显示出可产生一个物种或多个表现为一个物种的物种， 6-氨基喹啉基-N-羟基琥珀酰亚胺基氨基甲酸酯（AQC）。随着样品分子量的增加（Mr），需要增加试剂的摩尔过量。与天然物种相比，Tbe衍生产品表现出酸性p1位移，未经证实的峰效率和更低（提高）的检测限。在至少一种情况下，衍生产品（未完全标记）被Ab攻击时显示出对抗体（Ab）的识别，从而提高了在亲和力识别研究中使用这些衍生物的可能性-（例如，亲和力CE，免疫CE等）。在衍生化和聚焦过程中遇到沉淀问题。基本蛋白越多，这个问题就越明显。这似乎限制了该试剂作为clEF研究中通用的衍生试剂的适用性。本文介绍的结果代表了一种有前途的技术，它们提供了优势以及某些局限性。尽管尚不是完善的clEF中肽段分析和鉴定的完美方法，但这些结果表明了进一步优化的切实机会。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号