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首页> 外文期刊>Journal of liquid chromatography and related technologies >Analysis of maltodextrins in plant extracts by lc using evaporative light scattering edtection
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Analysis of maltodextrins in plant extracts by lc using evaporative light scattering edtection

机译:蒸发光散射检测-液相色谱法分析植物提取物中的麦芽糊精

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摘要

Two liquid chromatography methods using an evaporative light scattering detection (ELSD) have been developed to determine the carbohydrate profile of maltodextrins. The first method requires an octadecyl-bonded silica column and a methanol/water mobile phase. With the second method, an amino-bonded poly- meric stationary phase and an acetonitrile/water eluent are used. I.n the two cases, a gradient elution was necessary to desorb the higher molecular weight polysaccharides. This elution mode was perfectly compatible with ELSD since no baseline drift was observed. These two methods were applied to quantify mal- todextrins present in plant spray dried-powder of melilot and basil.
机译:已经开发出两种使用蒸发光散射检测(ELSD)的液相色谱方法来测定麦芽糊精的碳水化合物谱。第一种方法需要十八烷基键合的硅胶柱和甲醇/水流动相。在第二种方法中,使用了氨基键合的固定相和乙腈/水洗脱液。在这两种情况下,必须进行梯度洗脱才能解吸较高分子量的多糖。该洗脱模式与ELSD完全兼容,因为未观察到基线漂移。这两种方法被用于量化存在于梅洛特和罗勒的植物喷雾干粉中的麦芽糊精。

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