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首页> 外文期刊>Journal of liquid chromatography and related technologies >Versatile two-phase solvent system for flavonoid prefractionation by high-speed countercurrent chromatography
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Versatile two-phase solvent system for flavonoid prefractionation by high-speed countercurrent chromatography

机译：通用的两相溶剂体系，用于高速逆流色谱分离黄酮类化合物

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摘要

High-speed countercurrent chromatography was used for the prefractionation of flavonoids from the crude extracts of Pericarpium citri reticulatae, Radix puerariae, Radix glycyrrhizae, Radix scutellariae, and Flos genkwa. All separations were performed only with a two-phase system composed of CHCl3:CH3OH:H2O = 4:x:2. The x was changed for each sample between 2.5< similar-to >4.5. The fractionated components were identified by thinlayer chromatography, which confirmed this solvent system was versatile and very useful for prefractionation of flavonoids.
机译：高速逆流色谱法用于从柑桔皮，葛根，甘草，黄s和F药草的粗提物中对黄酮进行预分离。所有分离仅使用CHCl 3 ：CH 3 OH：H 2 O = 4：x：2组成的两相系统进行。对于每个样品，x在2.5 ＜相似至＞ 4.5之间变化。通过薄层色谱法鉴定了分离出的组分，这证实了该溶剂系统是通用的，对于黄酮类化合物的预分离非常有用。

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