...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Myoglobin immobilization on electrodeposited nanometer-scale nickel oxide particles and direct voltammetry.
【24h】

Myoglobin immobilization on electrodeposited nanometer-scale nickel oxide particles and direct voltammetry.

机译：肌红蛋白固定在电沉积的纳米级氧化镍颗粒上和直接伏安法。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Prosperity of information on the reactions of redox-active sites in proteins can be attained by voltammetric studies in which the protein sample is located on a suitable surface. This work reports the presentation of myoglobinickel oxide nanoparticles/glassy carbon (Mb/NiO NPs/GC) electrode, ready by electrochemical deposition of the NiO NPs on glassy carbon electrode and myoglobin immobilization on their surfaces by the potential cycling method. Images of electrodeposited NiO NPs on the surface of glassy carbon electrode were obtained by scanning electron microscopy (SEM) and atomic force microscopy (AFM). A pair of well-defined redox peaks for Mb(Fe(III)-Fe(II)) was obtained at the prepared electrode by direct electron transfer between the protein and nanoparticles. Electrochemical parameters of immobilized myoglobin such as formal potential (E(0')), charge transfer coefficient (alpha) and apparent heterogeneous electron transfer rate constant (k(s)) were estimated by cyclic voltammetry and nonlinear regression analysis. Biocatalytic activity was exemplified at the prepared electrode for reduction of hydrogen peroxide.
机译：可以通过伏安法研究获得蛋白质中氧化还原活性位点反应的信息的繁荣，其中将蛋白质样品放置在合适的表面上。这项工作报告了肌红蛋白/氧化镍纳米颗粒/玻璃碳（Mb / NiO NPs / GC）电极的介绍，该方法是通过将NiO NPs电化学沉积在玻璃碳电极上，并通过电势循环方法将肌红蛋白固定在其表面上来进行的。通过扫描电子显微镜（SEM）和原子力显微镜（AFM）获得了玻碳电极表面电沉积NiO NP的图像。通过在蛋白质和纳米粒子之间进行直接电子转移，在制备的电极上获得了一对Mb（Fe（III）-Fe（II））的明确定义的氧化还原峰。通过循环伏安法和非线性回归分析，估算了固定化肌红蛋白的电化学参数，如形式势（E（0'）），电荷转移系数（α）和表观异质电子转移速率常数（k（s））。在所制备的用于还原过氧化氢的电极上示例了生物催化活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号