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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Protection from Lyme neuroborreliosis in nonhuman primates with a multiantigenic vaccine.
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Protection from Lyme neuroborreliosis in nonhuman primates with a multiantigenic vaccine.

机译：使用多抗原疫苗在非人类灵长类动物中预防莱姆病引起的神经硼蛋白沉着症。

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In an effort to develop an effective and safe vaccine for lyme disease, rhesus macaques were injected with a multiantigenic preparation of Borrelia burgdorferi, strain N40. One month later animals were boosted before intradermal challenge with infectious B. burgdorferi. Challenges were performed at 1 and again at 5 months after the booster vaccination. Vaccinated and control nonvaccinated animals were monitored for development of systemic infection by measurement of serum anti-spirochetal antibodies by ELISA and Western blotting, and neurological involvement was monitored by testing of cerebrospinal fluid (CSF) and PCR analysis of central nervous system (CNS) tissue obtained at necropsy. Two control nonhuman primates (NHPs), given saline injections instead of vaccine and then challenged with B. burgdorferi, developed CSF pleocytosis, PCR positivity of the brain, and high levels of specific anti-B. burgdorferi antibody in the serum and CSF. Necropsy studies revealed widespread invasion of the CNS of one of these animals by the spirochete. In contrast, none of the four vaccinated animals developed evidence of invasion of the CNS after either of two challenge inoculations with infectious B. burgdorferi. In addition to resisting infection, no vaccinated animal demonstrated any untoward consequence of vaccination. These data demonstrate that a multiantigenic vaccine is effective in preventing systemic infection and lyme neuroboreliosis in NHPs and suggest that a successful vaccine could be developed in humans which would prevent lyme disease.
机译：为了开发一种用于莱姆病的有效和安全的疫苗，恒河猴被注射了博氏疏螺旋体N40株的多抗原制剂。一个月后，对动物进行感染，然后用感染性伯氏疏螺旋体进行皮内攻击。加强免疫后第1个月和第5个月再次进行攻击。通过ELISA和Western印迹法测量血清抗钩毛抗体来监测接种疫苗的和未接种的动物的全身感染的发生，并通过测试脑脊液（CSF）和中枢神经系统（CNS）组织的PCR分析来监测神经系统的受累情况尸检时获得。两个对照非人类灵长类动物（NHPs）注射盐水代替疫苗，然后用B. burgdorferi攻击，发展出CSF胞吞作用，大脑的PCR阳性和高水平的特异性抗B抗体。血清和CSF中的伯氏抗体。尸检研究表明，螺旋藻广泛侵袭了其中一种动物的中枢神经系统。相比之下，在用传染性伯氏疏螺旋体进行两次挑战接种后，四只接种的动物均未出现侵袭中枢神经的证据。除了抵抗感染外，没有疫苗的动物表现出任何不利的疫苗接种后果。这些数据表明，多抗原疫苗可有效预防NHPs的全身感染和莱姆病，并表明可以在人类中开发成功的疫苗来预防莱姆病。

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