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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Immunoblotting on HEp-2 cells increases the detection of antitopoisomerase 1 antibodies in patients with systemic sclerosis.
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Immunoblotting on HEp-2 cells increases the detection of antitopoisomerase 1 antibodies in patients with systemic sclerosis.

机译：对系统性硬化症患者的HEp-2细胞进行免疫印迹可提高抗拓扑异构酶1抗体的检测率。

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摘要

In order to improve the detection of antitopoisomerase 1 antibodies in a cohort of 111 systemic sclerosis patients, we have performed immunoblots on protein extracts of HEp-2 cells. Using indirect immunofluorescence and ELISA, 27 patients (24.3%) had antitopoisomerase 1 antibodies, 32 (28.8%) had anticentromere antibodies, 31 (27.9%) had antinuclear antibodies with none of these two antibodies and 21 (18.9%) had no antinuclear antibody. IgG from 24/27 (88.9%) patients with antitopoisomerase 1 antibodies bound to 2 protein bands of 63 and 100 kDa identified as topoisomerase 1 by N-terminal sequencing. Antitopoisomerase 1 antibodies were detected in 9 (8.1%) patients who had no antitopoisomerase 1 antibody as determined by ELISA. Patients with antitopoisomerase 1 antibodies had an almost similar phenotype without distinction between ELISA or immunoblot approaches. Our results provide evidence for the use of a combination of ELISA and immunoblot approaches for the detection of antitopoisomerase 1 antibodies.
机译：为了改善111名系统性硬化症患者队列中抗拓扑异构酶1抗体的检测，我们对HEp-2细胞的蛋白质提取物进行了免疫印迹分析。使用间接免疫荧光法和ELISA，27例（24.3％）的患者具有抗拓扑异构酶1抗体，32例（28.8％）的患者具有抗着丝粒抗体，31例（27.9％）的患者具有抗核抗体，而这两种抗体都不是，21例（18.9％）则没有抗核抗体。来自24/27（88.9％）患者的IgG具有抗拓扑异构酶1抗体，该抗体结合了63和100 kDa的2条蛋白带，通过N端测序鉴定为拓扑异构酶1。通过ELISA检测，在9名（8.1％）没有抗拓扑异构酶1抗体的患者中检测到抗拓扑异构酶1抗体。具有抗拓扑异构酶1抗体的患者具有几乎相似的表型，而在ELISA或免疫印迹方法之间没有区别。我们的结果为结合使用ELISA和免疫印迹方法检测抗拓扑异构酶1抗体提供了证据。

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