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首页> 外文期刊>Journal of Raman Spectroscopy: An International Journal for Original Work in All Aspects of Raman Spectroscopy, Including Higher Order Processes, and Also Brillouin- and Rayleigh Scattering >Experimental parameters for the SERS of nitrate ion for label-free semi-quantitative detection of proteins and mechanism for proteins to form SERS hot sites: A SERS study
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Experimental parameters for the SERS of nitrate ion for label-free semi-quantitative detection of proteins and mechanism for proteins to form SERS hot sites: A SERS study

机译：用于无标记半定量检测蛋白质的硝酸根离子SERS的实验参数和蛋白质形成SERS热点的机制：一项SERS研究

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摘要

We have explored the effects of the experimental parameters on the surface-enhanced Raman scattering (SERS) intensities of NO_3- and proteins observed by a heat-induced SERS method developed by our group. The results have shown that a strong SERS signal can be obtained at pH 4.0, using an Ag colloid prepared with the reduction time of 15 min (the average size of Ag nanoparticle is 56.5 nm) dilution prepared Ag colloid by a factor of 2 by use of a 5 mM citrate buffer, using 6 mM NaNO_3 and drying the sample at 100 °C, respectively. Based on the results, two possible mechanisms for proteins to form SERS hot sites during the sample preparations are proposed. A semi-quantitative SERS detection of ribonuclease B has been investigated. Also, NaNO_2, Mg (NO_3)_2, MgSO_4 and Na_2SO_4 have been found to be suitable for the heat-induced SERS method. Importantly, samples prepared by the heat-induced SERS method are so stable that these samples can be used as a standard and transferred to different laboratories for direct comparison. Namely, it can overcome uncontrollable aggregation of Ag colloids in a solution sample. All these advantages and the simplicity of experimental setup have demonstrated that the heat-induced SERS method using NaNO_3 as an electrolyte is very promising for label-free routine and quantitative detection of proteins.
机译：我们探索了实验参数对我们小组开发的热诱导SERS方法观察到的NO_3-和蛋白质表面增强拉曼散射（SERS）强度的影响。结果表明，使用还原时间为15分钟（Ag纳米颗粒的平均大小为56.5 nm）还原的15倍稀释制备的Ag胶体，使用pH值为2的倍数稀释，可以在pH 4.0下获得强SERS信号。使用6 mM NaNO_3洗脱5 mM柠檬酸盐缓冲液，并分别在100°C下干燥样品。根据结果​​，提出了两种在样品制备过程中蛋白质形成SERS热点的可能机制。研究了核糖核酸酶B的半定量SERS检测。而且，已经发现NaNO_2，Mg（NO_3）_2，MgSO_4和Na_2SO_4适合于热诱导的SERS方法。重要的是，通过热诱导SERS方法制备的样品非常稳定，可以用作标准样品，并转移到不同的实验室进行直接比较。即，它可以克服溶液样品中Ag胶体的不可控制的聚集。所有这些优点和实验设置的简单性证明，使用NaNO_3作为电解质的热诱导SERS方法对于无标记的常规和定量蛋白质检测非常有希望。

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