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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >ELISpot assay as a sensitive tool to detect cellular immunity following influenza vaccination in kidney transplant recipients.
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ELISpot assay as a sensitive tool to detect cellular immunity following influenza vaccination in kidney transplant recipients.

机译：ELISpot测定法是检测肾移植受体流感疫苗接种后细胞免疫力的灵敏工具。

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摘要

Enhanced cellular immunity following influenza vaccination has been undetectable in kidney transplant recipients so far. Protection from influenza is dependent on cellular and humoral immunity. Aim of the study was to investigate immune responses before and after vaccination with influenza A and B antigens in 65 kidney transplant recipients. A significant increase in proliferative responses was only observed towards influenza B (P < 0.0001) by lymphocyte transformation test. The enzyme-linked immunospot (ELISpot) assay was more sensitive and detected significant, 3- to 5-fold increases (P < 0.0001) in interferon-gamma secretion using influenza A and B antigens. Furthermore, influenza antibody titers increased significantly (P < 0.0001). At month 1 post-vaccination 85% of patients displayed specific cellular, and 95% or 92% humoral immunity against influenza A and B, respectively. Thus, applying the sensitive ELISpot assay, influenza-specific cellular immunity could be detected for the first time in kidney transplant recipients after vaccination.
机译：迄今为止，在肾脏移植接受者中未检测到流感疫苗接种后增强的细胞免疫性。预防流感取决于细胞和体液免疫。这项研究的目的是调查65位肾脏移植接受者在接种A和B流感抗原之前和之后的免疫反应。仅通过淋巴细胞转化试验观察到针对乙型流感的增殖反应显着增加（P <0.0001）。酶联免疫斑点法（ELISpot）测定更为灵敏，并使用甲型和乙型流感抗原检测到干扰素-γ分泌显着增加3至5倍（P <0.0001）。此外，流感抗体滴度显着增加（P <0.0001）。接种后第1个月，有85％的患者表现出针对甲型和乙型流感的特异性细胞免疫和95％或92％的体液免疫。因此，通过使用灵敏的ELISpot分析，可以在接种疫苗后的肾脏移植接受者中首次检测到流感特异性细胞免疫。

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