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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Immunofluorescence microscopy is superior to fluorescent beads for detection of antinuclear antibody reactivity in systemic lupus erythematosus patients.
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Immunofluorescence microscopy is superior to fluorescent beads for detection of antinuclear antibody reactivity in systemic lupus erythematosus patients.

机译：免疫荧光显微镜检查优于系统性红斑狼疮患者抗核抗体反应性的荧光珠。

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摘要

Antinuclear antibodies (ANA) are a hallmark of systemic lupus erythematosus (SLE) and one of its key diagnostic criteria. Recently, new technologies based on antibody binding to fluorescence beads (FB) have been widely employed for ANA screening. We conducted a formal study in 385 consecutive patients who underwent both traditional immunofluorescence (IF) and FB testing and evaluated each patient for the diagnosis of SLE. The distribution of ANA test results was significantly different (chi(2)=73.12; p<0.0001) due to a marked discordance of double-negative and double-positive results. The concordance of the FB-negative and IF-negative test results was 240/256 (95.6%), while the concordance of double-positive results was 54/129 (41.9%). The sensitivity of IF was markedly higher (48/53; 90.6%) than that of the FB (26/53; 49.1%; p<0.0001) for the diagnosis of SLE. IF had a lower specificity (76%) than FB (87%; p=0.0002). The present data show that the IF assay has superior sensitivity for detection of ANAand should continue to be used as the primary screening test for the diagnosis of SLE.
机译：抗核抗体（ANA）是系统性红斑狼疮（SLE）的标志，是其关键诊断标准之一。最近，基于抗体结合荧光珠（FB）的新技术已被广泛用于ANA筛选。我们对385名接受传统免疫荧光（IF）和FB检测的连续患者进行了正式研究，并评估了每位患者的SLE诊断。 ANA测试结果的分布存在显着差异（chi（2）= 73.12; p <0.0001），这是由于双阴性和双阳性结果之间存在显着差异。 FB阴性和IF阴性测试结果的一致性为240/256（95.6％），而双重阳性结果的一致性为54/129（41.9％）。对于SLE的诊断，IF的敏感性显着高于FB的敏感性（48/53; 90.6％）（26/53; 49.1％; p <0.0001）。 IF的特异性（76％）低于FB（87％; p = 0.0002）。目前的数据表明，IF分析对ANA的检测具有较高的灵敏度，应继续用作诊断SLE的主要筛选方法。

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