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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Dynamic changes of apoptosis-inducing ligands and Th1/Th2 like subpopulations in Hantaan virus-induced hemorrhagic fever with renal syndrome.
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Dynamic changes of apoptosis-inducing ligands and Th1/Th2 like subpopulations in Hantaan virus-induced hemorrhagic fever with renal syndrome.

机译：汉坦病毒引起的肾综合征出血热中凋亡诱导配体和Th1 / Th2样亚群的动态变化。

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摘要

The expression of the apoptosis-inducing ligands, TNF-alpha, FasL and TRAIL on peripheral blood mononuclear cells (PBMC) and the levels of their soluble form (TNF-alpha, sFasL and sTRAIL) in plasma from 40 hemorrhagic fever with renal syndrome (HFRS) patients as well as 26 healthy blood donors were determined by flow cytometry (FCM) analysis and sandwich ELISA, respectively. The status of Th1, Th2, Tc1 and Tc2 subsets in PBMC was evaluated by intracellular cytokine staining and FCM. Compared to controls, the expression of membrane bound FasL and TRAIL was up-regulated on surface of PBMC isolated from the HFRS patients, particularly on CD8+ T lymphocytes. The levels of TNF-alpha, sFasL and sTRAIL in plasma from the HFRS patients in the acute phase increase 4.7-fold, 6.0-fold and 1.8-fold, respectively, over those from the healthy donors. The percentage of Th1, Tc1 and Tc2 subsets in PBMC from the patients also increased significantly compared with those from healthy donors. These results indicate that dynamic changes occurred in both the membrane bound and soluble forms of apoptosis-inducing ligands (FasL, TRAIL and TNF-alpha) and proportions of Th1 and CTL in HFRS patients increased. Both factors may play an important role in the etiology of Hantaan virus infection in humans.
机译：40例肾综合征出血热患者血浆中诱导凋亡的配体TNF-α，FasL和TRAIL在外周血单个核细胞（PBMC）中的表达及其可溶形式（TNF-α，sFasL和sTRAIL）的水平（通过流式细胞术（FCM）分析和三明治ELISA分别确定了HFRS）患者和26位健康献血者。通过细胞内细胞因子染色和FCM评估PBMC中Th1，Th2，Tc1和Tc2子集的状态。与对照相比，从HFRS患者分离的PBMC的表面，特别是在CD8 + T淋巴细胞上，膜结合的FasL和TRAIL的表达上调。 HFRS患者急性期血浆中的TNF-α，sFasL和sTRAIL的水平分别比健康供体的高4.7倍，6.0倍和1.8倍。与健康捐献者相比，患者PBMC中Th1，Tc1和Tc2亚群的百分比也显着增加。这些结果表明，在HFRS患者中，膜结合形式和可溶性形式的凋亡诱导配体（FasL，TRAIL和TNF-α）均发生了动态变化，Th1和CTL的比例增加。两种因素都可能在人类汉坦病毒感染的病因中起重要作用。

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