• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Expression of costimulatory molecules CD80 and/or CD86 by a Kaposi's sarcoma tumor cell line induces differential T-cell activation and proliferation.
【24h】

Expression of costimulatory molecules CD80 and/or CD86 by a Kaposi's sarcoma tumor cell line induces differential T-cell activation and proliferation.

机译：卡波西氏肉瘤肿瘤细胞系表达共刺激分子CD80和/或CD86可诱导差异性T细胞活化和增殖。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

During physiological stimulation of resting T-cells, at least two activation signals by antigen presenting cells are required. Besides the first antigen-specific signal, the second costimulatory signal involves CD80 and CD86 expressed by the antigen presenting cell. These costimulatory molecules have been suggested to be of clinical relevance in many different autoimmune and malignant disease processes. We previously observed that tumor cells in Kaposi's sarcoma (a common AIDS-related cutaneous neoplasm) completely lack both CD80 and CD86, and these tumor cells fail to stimulate T-cell proliferation. In this study, using a Kaposi's sarcoma tumor cell line designated SLK, various stable transfected cell lines were produced. Tumor cells that were either singly positive for either CD80 or CD86, as well as a double-positive cell line, were examined for their ability to induce T-cell activation, T-cell proliferation, and cytokine production profiles. Compared to the parental double-negative tumor cell line, the CD80-positive cells, but not the CD86-positive tumor cells, induced significant T-cell activation and proliferation. Tumor cells expressing both CD80 and CD86 also induced T-cell activation. After stimulation by the transfected tumor cells, T-cells produced a Th-1 type cytokine production profile with increased IL-2 and IFN-gamma levels. These results demonstrate that Kaposi's sarcoma tumor cells lacking co-stimulatory molecules cannot induce T-cell activation; however, if they express CD80, they can induce peripheral blood T-cell proliferation, and there is a differential response as expression of CD86 did not have the same immunostimulatory effect.
机译：在对静止的T细胞进行生理刺激期间，需要至少两个抗原呈递细胞的激活信号。除了第一抗原特异性信号之外，第二共刺激信号还涉及抗原呈递细胞表达的CD80和CD86。这些共刺激分子已被认为在许多不同的自身免疫和恶性疾病过程中具有临床意义。我们先前观察到卡波济肉瘤（一种常见的AIDS相关皮肤肿瘤）中的肿瘤细胞完全缺乏CD80和CD86，并且这些肿瘤细胞无法刺激T细胞增殖。在这项研究中，使用称为SLK的卡波西氏肉瘤肿瘤细胞系，生产了各种稳定的转染细胞系。检查对CD80或CD86单阳性的肿瘤细胞，以及双阳性细胞系，它们诱导T细胞活化，T细胞增殖和细胞因子产生的能力。与亲代双阴性肿瘤细胞系相比，CD80阳性细胞而非CD86阳性肿瘤细胞诱导了显着的T细胞活化和增殖。同时表达CD80和CD86的肿瘤细胞也诱导T细胞活化。在被转染的肿瘤细胞刺激后，T细胞产生Th-1型细胞因子，其IL-2和IFN-γ水平升高。这些结果表明，缺乏共刺激分子的卡波济氏肉瘤肿瘤细胞不能诱导T细胞活化。但是，如果它们表达CD80，则可以诱导外周血T细胞增殖，并且存在差异应答，因为CD86的表达没有相同的免疫刺激作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号