• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >C/EBP beta in rheumatoid arthritis: correlation with inflammation, not disease specificity.
【24h】

C/EBP beta in rheumatoid arthritis: correlation with inflammation, not disease specificity.

机译：类风湿关节炎中的C / EBP beta：与炎症相关，而非疾病特异性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Rheumatoid arthritis synovial tissue was examined and compared with osteoarthritis tissue for the presence of the nuclear transcription factor C/EBP beta (NF-IL-6). The region (lining or sublining), cell type, and subcellular distribution (cytoplasmic or nuclear) of the expression of C/EBP beta was characterized. Rheumatoid arthritis synovial fluid and blood and normal peripheral blood were also examined. C/EBP beta was detected in the synovial lining and in sublining cells of synovial tissue from patients with both rheumatoid and osteoarthritis. A significant (P < 0.001 and < 0.05, respectively) increase in the percentage of cells with nuclear staining was seen in the lining layer, compared to cells in the sublining region, in rheumatoid and osteoarthritis. In both diseases a strong correlation (r = 0.79, P < 0.001) was observed between the percentage of cells in the synovial lining that were positive for nuclear C/EBP beta and lining cell depth. Two-color immunohistochemistry demonstrated that both macrophages and fibroblast-like synoviocytes were positive for nuclear C/EBP beta. The presence of C/EBP beta was confirmed by immunohistochemistry and Western blot analysis with isolated synovial fibroblasts. Nuclear C/EBP beta was also detected in rheumatoid synovial fluid monocytes/macrophages, but not in lymphocytes or neutrophils. Western blot analysis confirmed the presence of C/EBP beta in these cells. The intensity of C/EBP beta staining was greater (P < 0.001) in synovial fluid monocytes than in those from normal or rheumatoid peripheral blood. In conclusion, the enhanced nuclear staining for C/EBP beta in the synovial lining, compared to the sublining, suggesting activation in the lining, and the positive correlation of lining layer depth with the percentage of cells in the lining positive for nuclear C/EBP beta, suggest a potential role for C/EBP beta in chronic inflammation. The regulation of the production or activity of C/EBP beta, to inhibit inflammatory mediator expression by synovial macrophages and fibroblasts, offers a novel approach to therapeutic intervention.
机译：检查了类风湿关节炎滑膜组织，并与骨关节炎组织进行了核转录因子C / EBPβ（NF-IL-6）的比较。表征了C / EBP beta表达的区域（衬里或亚衬里），细胞类型和亚细胞分布（细胞质或细胞核）。还检查了类风湿关节炎滑液，血液和正常外周血。在类风湿和骨关节炎患者的滑膜衬里和滑膜组织的亚细胞中均检测到C / EBP beta。在类风湿和骨关节炎中，与下层区域的细胞相比，内层的细胞核染色百分比显着增加（分别为P <0.001和<0.05）。在这两种疾病中，观察到滑膜衬层中核C / EBPβ阳性的细胞百分比与衬层细胞深度之间有很强的相关性（r = 0.79，P <0.001）。两种颜色的免疫组织化学表明，巨噬细胞和成纤维细胞样滑膜细胞均对核C / EBP beta呈阳性。通过免疫组织化学和分离的滑膜成纤维细胞的Western印迹分析证实了C / EBP beta的存在。在类风湿性滑液单核细胞/巨噬细胞中也检测到核C / EBPβ，但在淋巴细胞或中性粒细胞中未检测到。蛋白质印迹分析证实了这些细胞中存在C / EBP beta。滑液单核细胞中C / EBPβ染色的强度大于正常或类风湿性外周血中的C / EBPβ染色强度（P <0.001）。总之，与亚衬层相比，滑膜衬层中C / EBPβ的核染色增强，表明衬层中的活化，并且衬层深度与衬层中细胞的百分比呈正相关，呈核C / EBP阳性β，提示C / EBP beta在慢性炎症中的潜在作用。抑制滑膜巨噬细胞和成纤维细胞抑制炎症介质表达的C / EBPβ产生或活性的调节，为治疗干预提供了一种新颖的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号