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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Immunological approach in the evaluation of regional lymph nodes of patients with squamous cell carcinoma of the head and neck.
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Immunological approach in the evaluation of regional lymph nodes of patients with squamous cell carcinoma of the head and neck.

机译：免疫学方法评估头颈部鳞状细胞癌患者的区域淋巴结。

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In cancer, regional lymph node (LN) cells are one of the first components of the immune system to have contact with tumor cells or their products. Therefore, the phenotype and functional properties of hematopoietic cells present within the tumor-draining LN are important to understanding their role in the control of malignant cells. Based on the locoregional metastatic behavior of squamous cell carcinoma of the head and neck (SCCH&N) region, we analyzed tumor-draining lymph nodes from SCCH&N patients to obtain insights into regional tumor immunity. Using a three-color fluorescent labeling technique, surface antigen expression was visualized in mononuclear cells of lymph nodes that were obtained from head and neck cancer patients and compared to mononuclear cells of normal lymph nodes. Cell cycle analyses were performed using propidium iodide. Proliferation after phytohemagglutinin stimulation was measured by a sodium tetrazolium-based assay. LN histology was correlated with flow cytometric findings. Regional lymph nodes of head and neck cancer patients undergo morphologic and functional changes. Flow cytometry revealed a decrease in CD8(+) T cells and in some lymph nodes the presence of second or third populations of larger cells with distinct size and granularity that expressed both T (gammadelta/alphabeta) and different natural killer cell markers. Moreover, cell cycle analyses and proliferation assays showed a diminished response to mitogenic stimuli. These changes were found in both metastatic and hyperplastic lymph nodes from head and neck cancer patients; however, no alterations were found in control lymph nodes or peripheral blood mononuclear cells from noncancer patients. The immune alterations detected in lymphocytes present within the draining lymph nodes of head and neck cancer patients may improve our understanding of how tumor cells escape host immunosurveillance. However, this dysfunction in local draining lymph nodes may not be detected systemically. (c)2001 Elsevier Science.
机译：在癌症中，区域淋巴结（LN）细胞是免疫系统与肿瘤细胞或其产物接触的第一批成分之一。因此，存在于引流液的LN中的造血细胞的表型和功能特性对于理解其在恶性细胞控制中的作用很重要。基于头颈部鳞状细胞癌（SCCH＆N）区域的局部转移行为，我们分析了SCCH＆N患者的引流淋巴结，以了解区域肿瘤免疫力。使用三色荧光标记技术，在从头颈癌患者获得的淋巴结单核细胞中可视化表面抗原表达，并将其与正常淋巴结的单核细胞进行比较。使用碘化丙啶进行细胞周期分析。植物血凝素刺激后的增殖通过基于四唑钠的测定来测量。 LN组织学与流式细胞术发现相关。头颈癌患者的区域淋巴结发生形态和功能变化。流式细胞仪显示CD8（+）T细胞的减少，在某些淋巴结中，存在第二个或第三个较大细胞，它们具有不同的大小和粒度，可同时表达T（γ/γ）和不同的自然杀伤细胞标记。此外，细胞周期分析和增殖测定表明对促有丝分裂刺激的应答减弱。在头颈癌患者的转移性和增生性淋巴结中均发现了这些变化。然而，在非癌症患者的对照淋巴结或外周血单个核细胞中未发现任何改变。在头颈癌患者的引流淋巴结内存在的淋巴细胞中检测到的免疫改变可能会增进我们对肿瘤细胞如何逃脱宿主免疫监视的了解。但是，这种局部引流淋巴结功能障碍可能无法被系统检测到。 （c）2001 Elsevier科学。

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