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首页> 外文期刊>Journal of Reproductive Immunology >Lymphocyte phenotyping and NK cell activity analysis in pregnant NOD/SCID mice.
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Lymphocyte phenotyping and NK cell activity analysis in pregnant NOD/SCID mice.

机译：怀孕的NOD / SCID小鼠的淋巴细胞表型和NK细胞活性分析。

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The purpose of this study was to outline the fertility features of non-obese diabetic (NOD)/LtSz-scid/scid (NOD/SCID [severe combined immunodeficiency] for short) mice, and to evaluate the effects of NK cell subsets on the pregnancy outcomes of the syngeneic NOD/SCID x NOD/SCID mating combination. Firstly, lymphocyte phenotyping was performed with flow cytometry to confirm the multiple immunodeficits in NOD/SCID mice. Fertility features were assessed in NOD/SCID x NOD/SCID mice and compared with non-immunodeficiency control BALB/c x BALB/c mice. Although the presence of NK cell deficit is apparent in NOD/SCID mice, a certain level of remnant NK activity could be observed in these mice. The remnant NK cell activity was stimulated with polyinosinic-polycytidylic acid (polyIC), or inhibited with anti-asialo GM1 (ASGM1) anti-serum, respectively. The effects of these factors on pregnancy outcomes were evaluated after administration. Roughly normal fertility could be observed in NOD/SCID x NOD/SCID mice. However, a slightly larger sized litter was observed in polyIC-treated NOD/SCID x NOD/SCID mice compared with control NOD/SCID mice. In contrast, embryo resorption was boosted after ASGM1 injection, and correlated subsequently with a smaller litter size. It indicates that the remnant NK cell activity in NOD/SCID mice may be beneficial to feto-maternal tolerance during pregnancy.
机译：这项研究的目的是概述非肥胖糖尿病（NOD）/ LtSz-scid / scid（简称NOD / SCID [简称为严重联合免疫缺陷]）小鼠的生育能力，并评估NK细胞亚群对小鼠的影响。同种NOD / SCID x NOD / SCID交配组合的妊娠结局。首先，用流式细胞仪进行淋巴细胞表型分析，以确认NOD / SCID小鼠的多种免疫缺陷。在NOD / SCID x NOD / SCID小鼠中评估了生育能力，并与非免疫缺陷对照BALB / c x BALB / c小鼠进行了比较。尽管在NOD / SCID小鼠中明显存在NK细胞缺陷，但在这些小鼠中可以观察到一定水平的残余NK活性。残余的NK细胞活性分别由多肌苷酸-聚胞苷酸（polyIC）刺激，或由抗-亚洲人GM1（ASGM1）抗血清抑制。给药后评估这些因素对妊娠结局的影响。在NOD / SCID x NOD / SCID小鼠中可以观察到大约正常的生育力。但是，与对照NOD / SCID小鼠相比，在经polyIC处理的NOD / SCID x NOD / SCID小鼠中观察到大小稍大的窝。相比之下，注射ASGM1后胚胎的吸收增强，并且随后与较小的窝数相关。这表明NOD / SCID小鼠中残留的NK细胞活性可能对怀孕期间的胎儿母体耐受性有益。

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