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首页> 外文期刊>Journal of Reproductive Immunology >Integrin alpha5 is involved in fibronectin-induced human extravillous trophoblast invasion.
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Integrin alpha5 is involved in fibronectin-induced human extravillous trophoblast invasion.

机译：整联蛋白α5参与纤连蛋白诱导的人类绒毛外滋养层细胞入侵。

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摘要

To identify the molecules involved in human extravillous trophoblast (EVT) invasion, we raised murine mAbs that react with EVTs and obtained one mAb (CHL3) that inhibited invasion of a human choriocarcinoma-derived cell line, BeWo cells. The N-terminal 22 aminoacid sequence of the CHL3 antigen (150kDa) purified from placental tissue completely matched that of integrin alpha5, which is known to interact with fibronectin. Double immunohistochemical staining and flow cytometry confirmed the reactivity of CHL3 with integrin alpha5 and its expression on the surface of BeWo cells and human EVTs isolated from villous explant cultures. CHL3 mAb inhibited the attachment of human EVTs and BeWo cells to fibronectin-coated dishes, but not to Matrigel dishes. In the Matrigel invasion assay supplemented with or without fibronectin, the invasion of isolated EVTs and BeWo cells was attenuated by treatment with CHL3 without affecting cell proliferation. During invasion assays, the production of matrix metalloproteases 2 and 9 was not changed by CHL3. These findings suggest that interaction with fibronectin through integrin alpha5 plays an important role in human extravillous trophoblast invasion.
机译：为了鉴定参与人类绒毛外滋养层细胞（EVT）侵袭的分子，我们提出了与EVT反应的鼠单克隆抗体，并获得了一种单克隆抗体（CHL3），该抗体可抑制人绒毛膜癌衍生的细胞系BeWo细胞的侵袭。从胎盘组织中纯化的CHL3抗原（150kDa）的N端22个氨基酸序列与整合素α5完全匹配，后者已知与纤连蛋白相互作用。双重免疫组织化学染色和流式细胞术证实CHL3与整联蛋白alpha5的反应性及其在BeWo细胞和从绒毛外植体培养物中分离的人EVT的表面上的表达。 CHL3 mAb抑制人EVT和BeWo细胞附着在纤连蛋白包被的培养皿上，但不附着在Matrigel培养皿上。在补充或不加入纤连蛋白的基质胶侵袭试验中，用CHL3处理可减轻分离的EVT和BeWo细胞的侵袭，而不会影响细胞增殖。在入侵试验中，CHL3不会改变基质金属蛋白酶2和9的产生。这些发现表明通过整联蛋白α5与纤连蛋白的相互作用在人类绒毛外滋养层细胞入侵中起重要作用。

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