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首页> 外文期刊>Journal of Reproductive Immunology >Placental-specific microRNA in maternal circulation--identification of appropriate pregnancy-associated microRNAs with diagnostic potential.
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Placental-specific microRNA in maternal circulation--identification of appropriate pregnancy-associated microRNAs with diagnostic potential.

机译：孕妇循环中的胎盘特异性microRNA-鉴定具有诊断潜力的适当的妊娠相关microRNA。

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The goal of this study was to identify placental specific microRNAs present in maternal plasma that differentiate between women with normal pregnancies and nonpregnant individuals. The selection of appropriate pregnancy-associated microRNAs with diagnostic potential was based on the following criteria: (1) detection rate of 100% in full-term placentas, (2) detection rate of >/= 67% in maternal plasma throughout gestation (at least four positive wells out of six tested wells) and (3) not detectable in whole peripheral blood and plasma samples of nonpregnant individuals. Initially, we tested microRNAs (miR-34c, miR-372, miR-135b and miR-518b), which had been previously identified as pregnancy-associated microRNAs. Additionally, we selected 16 other highly specific placental microRNAs (miR-512-5p, miR-515-5p, miR-224, miR-516-5p, miR-517*, miR-136, miR-518f*, miR-519a, miR-519d, miR-519e, miR-520a*, miR-520h, miR-524-5p, miR-525, miR-526a, and miR-526b) from the miRNAMap database. Seven microRNAs (miR-516-5p, miR-517*, miR-518b, miR-520a*, miR-520h, miR-525 and miR-526a) were identified as new pregnancy associated microRNAs with diagnostic potential. Their levels in maternal plasma during the 36th week of gestation corresponded to 45.0-427.0 pg of total RNA (enriched for small RNAs) per milliliter of maternal plasma.
机译：这项研究的目的是鉴定母体血浆中存在的胎盘特异性microRNA，以区分正常怀孕的妇女和未怀孕的妇女。根据以下标准选择具有诊断潜能的合适的妊娠相关microRNA：（1）足月胎盘的检出率为100％，（2）整个孕期（在妊娠时）母体血浆的检出率为> / = 67％在六个测试孔中，至少有四个阳性孔）和（3）在非怀孕个体的全血和血浆样本中无法检测到。最初，我们测试了microRNA（miR-34c，miR-372，miR-135b和miR-518b），它们先前已被鉴定为与怀孕相关的microRNA。此外，我们选择了16种其他高度特异性的胎盘microRNA（miR-512-5p，miR-515-5p，miR-224，miR-516-5p，miR-517 *，miR-136，miR-518f *，miR-519a ，miR-519d，miR-519e，miR-520a *，miR-520h，miR-524-5p，miR-525，miR-526a和miR-526b）。七个微RNA（miR-516-5p，miR-517 *，miR-518b，miR-520a *，miR-520h，miR-525和miR-526a）被鉴定为具有诊断潜力的新妊娠相关microRNA。在妊娠第36周，它们在母体血浆中的水平相当于每毫升母体血浆45.0-427.0 pg总RNA（富含小RNA）。

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