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首页> 外文期刊>Journal of Reproductive Immunology >Effects of candidate vaginally-applied microbicide compounds on innate immune cells.
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Effects of candidate vaginally-applied microbicide compounds on innate immune cells.

机译：阴道应用的杀菌剂对先天免疫细胞的影响。

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Ideally, a vaginally-applied microbicide would be effective against a broad range of pathogens but would have minimal effects on the female genital tract. The aim of this study was to determine if representative candidate detergent-type and sulfated/sulfonated polymer-type microbicides altered the composition or function of innate immune cells normally found in the vaginal mucosa. The effect of microbicide on the composition of vaginal leukocytes was tested using a flow cytometric approach. Application of the detergent cholic acid, but not the sulfated polysaccharide lambda carrageenan, resulted in a significant increase in macrophages at the vaginal epithelial surface compared to control treatment (19.3% macrophages compared to 2.8%; p<0.0004). Phagocytosis of fluorochrome-labeled bacteria by macrophages was inhibited greater than 50% in the presence of 1.0mg/ml of the sulfonated polymer PRO 2000 but was not inhibited by the same concentration of lambda carrageenan. PRO 2000-pulsed macrophages regained phagocytic function after being washed free of the compound. Culture of macrophages with PRO 2000 also resulted in diminished detection of the surface proteins CD11b and CD18. After treated cells were washed free of PRO 2000, these proteins were detected at levels similar to control treated cells. In conclusion, application of a detergent-type microbicide, but not a sulfated polymer, resulted in the infiltration of inflammatory cells at the vaginal epithelial surface. Phagocytic function of macrophages was lost in the presence of 1mg/ml PRO 2000 which may have reflected masking of important cell surface proteins by the microbicide; however, there was no evidence of permanent loss of function upon removal of the compound.
机译：理想地，阴道施用的杀微生物剂将对多种病原体有效，但对女性生殖道的影响极小。本研究的目的是确定代表性的去污剂型和硫酸化/磺化聚合物型杀菌剂是否会改变通常在阴道粘膜中发现的先天免疫细胞的组成或功能。使用流式细胞术测试了杀菌剂对阴道白细胞组成的影响。与对照处理相比，洗涤剂胆酸的施用而不是硫酸化多糖λ-角叉菜胶的施用导致了巨噬细胞在阴道上皮表面的显着增加（巨噬细胞为19.3％，而2.8％; p <0.0004）。在1.0mg / ml磺化聚合物PRO 2000的存在下，巨噬细胞对荧光染料标记的细菌的吞噬作用抑制大于50％，但不受相同浓度的λ角叉菜胶的抑制。冲洗PRO 2000脉冲的巨噬细胞后，该化合物恢复了吞噬功能。用PRO 2000培养巨噬细胞也导致表面蛋白CD11b和CD18的检测减少。将处理过的细胞洗去PRO 2000后，检测到的这些蛋白的水平与对照处理过的细胞相似。总之，使用洗涤剂型杀微生物剂而非硫酸化聚合物可导致阴道上皮表面的炎症细胞浸润。在存在1mg / ml PRO 2000的情况下，巨噬细胞的吞噬功能丧失了，这可能反映了杀菌剂对重要细胞表面蛋白的掩盖作用。但是，没有证据表明去除该化合物后永久丧失功能。

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