• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Neutralizing monoclonal antibodies to the E2 protein of chikungunya virus protects against disease in a mouse model
【24h】

Neutralizing monoclonal antibodies to the E2 protein of chikungunya virus protects against disease in a mouse model

机译：针对基孔肯雅病毒E2蛋白的中和性单克隆抗体可预防小鼠模型中的疾病

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Chikungunya virus (CHIKV) recently caused the largest epidemic ever recorded for this virus involving an estimated 1.4-6.5 million cases, with imported cased reported in over 40 countries. The number of monoclonal antibodies specific for this re-emerging alphavirus is currently limited. Herein we describe the generation and characterisation of five monoclonal antibodies specific for the E2 glycoprotein of CHIKV. The antibodies detected a range of CHIKV isolates in several assays including ELISA, Western blot, immunofluorescence assay (IFA) and immunohistochemistry (IHC) without evidence of cross-reactivity with other alphaviruses. Four antibodies also neutralised CHIKV in vitro, two of which provided complete protection against arthritis in a CHIKV mouse model when administered prior to infection. Given the current shortage of widely available reagents for CHIKV, these specific antibodies will be useful not only in research, but may also provide the basis for new diagnostics and treatments.
机译：摘要基孔肯雅病毒（CHIKV）最近引起了有史以来最大的流行病记录，估计有140至650万例，其中40多个国家报告了进口病例。目前对于这种重新出现的α病毒特异的单克隆抗体的数量受到限制。在本文中，我们描述了CHIKV的E2糖蛋白特异的五种单克隆抗体的产生和表征。该抗体在包括ELISA，Western blot，免疫荧光测定（IFA）和免疫组织化学（IHC）在内的数种检测方法中检测到一系列CHIKV分离株，而没有与其他α病毒交叉反应的证据。四种抗体还可以在体外中和CHIKV，其中两种在感染前给药后可在CHIKV小鼠模型中提供针对关节炎的完全保护。鉴于目前缺乏用于CHIKV的广泛可用的试剂，这些特异性抗体不仅将在研究中有用，而且可能为新的诊断和治疗方法提供基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号